扶正抑瘤方含药血清对PC-3细胞作用的实验研究

Effects of serum containing Fuzhengyiliu Fang on PC-3 human prostate cancer cell

目的:通过体外细胞实验,探讨扶正抑瘤方有效治疗前列腺癌的作用机理。方法:以人前列腺癌PC-3细胞为模型,观察扶正抑瘤方高、中、低剂量(9.58、19.17、38.33 g/kg)及不同浓度含药血清对PC-3细胞形态、细胞增殖、细胞周期及细胞凋亡的影响。结果:扶正抑瘤方含药血清处理后,PC-3细胞可发现不同程度的细胞皱缩,染色质凝集,或细胞核固缩碎裂;MTT实验表明含药血清能明显抑制PC-3细胞增殖,且剂量越高细胞增殖抑制率越高,20%高剂量含药血清组抑制率约50%;流式细胞仪检测发现,含药血清组处理后48 h,各组在非凋亡细胞二倍体峰(G1)之前均出现大小不等的凋亡细胞峰,凋亡率均显著高于空白血清组,而且G0/G1期细胞比例增加,S期及G2/M期细胞比例下降。结论:扶正抑瘤方含药血清能抑制PC-3细胞增殖,且呈剂量、浓度依赖性,其抗肿瘤活性可能与诱导肿瘤细胞凋亡有关。

AIM：To observe the effective mechanism of Fuzhengyiliu Fang（FZF） in the treatment of prostate cancer in vitro. METHODS：Serum of high,medium,low doses of FZF and control groups were prepared by serum pharmacology methods,human prostate cancer PC-3 cells applied as an experiment model,this study observed morphology,inhibition rate of cell proliferation,cell cycle and cell apoptosis of PC-3 cells affected by different doses and concentrations of FZF serum.RESULTS：After treated with FZF serum,PC-3 cells were found in different degrees of cell shrinkage,chromatin condensation,or nuclear condensation and fragmentation;MTT experiment showed FZF serum obviously restrained cell proliferation,and the higher the concentration,the higher the rate of inhibition,20% high-dose FZF containing serum group inhibited cell proliferation about 50%;flow cytometry detected that after treated 48 h,different sizes of the apoptotic peak had appeared before the diploid peak（G1） of non-apoptotic PC-3 cells,and the apoptosis rate of all treated groups were significantly higher than the control group,the proportion of G0/G1 phase cells increased,S and G2/M phase cells decreased.CONCLUSION：FZF serum can inhibit proliferation of PC-3 cells in dose,concentration-dependent trend,this activity may be related with its effect of inducing tumor cell apoptosis,which provide an important experimental basis for the clinical treatment of prostate cancer with FZF.