早中期食管鳞状细胞癌中Notch1与血管内皮生长因子C的表达及其意义

Expression of Notchl and vascular endothelial growth factor C and its significance in early metaphase esophageal squamous cell carcinoma

目的研究Notchl与血管内皮生长因子C（VEGF-C）在早中期食管鳞状细胞癌（ESCC）中的表达及其与临床病理特征的关系。方法收集2007年5月至12月在本院行食管癌根治术的40例早中期ESCC和8例正常食管组织病理标本，免疫组化方法检测Notch1和VEGF-C的表达，并探讨其与临床病理特征的关系，分析Notchl和VEGF-C表达的相关性。结果与正常食管组织比较，早中期ESCC组织Notchl表达阳性率下降[47．5％（19／40）比100．0％（8／8），P=0．006）]，而VEGF—C表达升高[67．5％（27／40）比0．0％（0／8），P=0．000]。高、中、低分化ESCC组织中Notchl表达阳性率分别为80．0％（8／10）、50．0％（7／14）、25．0％（4／16），Notchl表达阳性率随肿瘤分化程度降低而降低（P=0．023），但在肿瘤浸润程度和淋巴结转移中差异无统计学意义（均P〉0．05）。高、中、低分化ESCC组织中VEGF—C表达阳性率分别为30．0％（3／10）、71．4％（10／14）、81．3％（13／16），VEGF-C表达阳性率随分化程度的降低而升高（P=0．024）。淋巴结转移阳性组VEGF—C表达阳性率高于淋巴结转移阴性组[100．0％（16／16）比45．8％（11／24），P=0．000]。与T2分期组比较，T3～T4分期组ESCC组织VEGF—C表达阳性率升高[82．1％（23／28）比33．3％（4／12），P=0．000]。Notchl与VEGF—C表达呈负相关（r=0．486，P=0．003）。结论Notchl在早中期ESCC中表现为抑癌基因，其异常低表达可能是引起VEGF—C异常高表达的因素之一。

Objective To study the expression of Notchl and vascular endothelial growth factor C （VEGF-C） in early metaphase esophageal squamous cell carcinoma （ESCC） and its relationship with the clinicopathological characters. Methods Tissue slices of 40 ESCC and 8 normal esophagus tissues were collected during esophagectomy in our hospital from May 2007 to December 2007. The expression of Notchl and VEGF-C was detected by immunohistochemistry. The correlation between Notchl and VEGF-C and the relationship with the clinicopathological characters were discussed. Results Compared in early metaphase ESCC to that in normal tissue, Notch I was obviously low expressed [ 47.5% （19/40） vs 100.0% （8/8）, P=0.006） ], and VEGF-C was high expressed [ 67.5% （ 27/40 ） vs 0.0% （0/8）, P=0.000 ]. Notch 1 expression rate in different phase ESCC was 80.0% （ 8/10, well-differentiated）, 50.0% （7/14, moderate-differentiated）, 25.0% （4/16, poor-differentiatedwhile）, respectively. The positive expression rate of Noehl decreased with the decrease of differentiation degree （P= 0.023 ）, but there was no significant differences between infiltration degree and lymph node metastasis（P〉0.05 ）. While VEGF-C expression rate was 30.0% （ 3/10, well-differentiated）, 71.4% （ 10/14, moderate-differentiated）, 81.3% （13/16, poor-differentiated）, respectively. The expression of VEGF-C increased with the decrease of differentiation degree（P=0.024）. VEGF-C expression in lymph nodes metastasis group was higher than that in no lymph nodes metastasis group [ 100.0% （16/16） vs 45.8% （11/24） ,P=0.000]. Compared with T2 group, the positive rate of VEGF-C expression was higher in T3-T4 group in ESCC tissue [ 82.1% （23/28） vs 33.3% （4/12）, P=0.000 ]. Correlation was negative between Notchl and VEGF-C （r=-0.486, P=0.003 ）. Conclusions Notch 1 may be one of the anti-oncogenes in early metaphase ESCC. The aberrant low expression of Nochl may lead to aberrant high expression of VEGF-C.