当归注射液对急性肺栓塞大鼠P-选择素、抗心磷脂抗体的作用

The role of angelica injection on P-selectin and anti-cardiolipin antibodies in acute pulmonary embolism rats

目的:研究当归注射液对急性肺栓塞大鼠P-、E-选择素及抗心磷脂抗体(ACA)表达的影响。方法:SD大鼠按完全随机设计分为正常对照组(N组),血栓栓塞组(T组),当归治疗组(TA组),各组分1h、4h、8h3个时间点,分别用ELISA、免疫组化法检测大鼠P-、E-选择素及ACA水平。结果:肺组织HE染色N组大鼠肺内炎症细胞数较少。T组、TA组大鼠肺内炎症细胞数分别较N组升高(P<0.05),且T组大鼠肺内炎症细胞数随时间延长逐渐增多;血浆ELISA法检测P-、E-选择素结果:T组分别较N组表达增高(P<0.05);TA组分别较T组各相应时间点表达降低(P<0.05);各组间大鼠血浆ACA的OD值无统计学意义(P>0.05),但免疫组化显示TA组大鼠肺组织的P-,E-选择素及ACA表达均降低。结论:急性肺栓塞可引起大鼠肺部炎症细胞浸润,并可能通过P-、E-选择素及ACA释放增加,释放炎症介质加重肺损伤;中药当归注射液可能通过抑制P-、E-选择素及ACA的表达,减轻急性肺栓塞大鼠肺部炎症反应从而减少血栓形成。

Objective：To study the effect of chinese medicine, Angelica, injection on the expression of P-, E-selectin and anti-cardiolipin antibody in acute pulmonary embolism rats. Methods： SD rats were randomly divided into 3 groups： normal control group（Group N）, thromboembolism group（Group T）, and treatment group of thromboembolism with angelica injection（Group TA）.There were three time points in every group：1 h, 4 h and 8 h. Plasma was detected by P-, with 4% paraformaldehyde,and paraffin embedded sections were detected by immunohistochemistry for P-, E-selectin and anti-cardiolipin antibodies. Results： With HE stain, the inflammatory cells in the lung of rats were relatively rare in every time point in normal control group. In group T and group TA, the inflammatory cells were increasing in every time point in comparison to group N（P0.05） and the inflammatory cells were increasing with time in group T. The data revealed that the plasmic level of P-,E-selectin was significantly higher than that in group T1, group T4, group T8 in comparison to the corresponding sub groups of group N（P0.05）, while it was significantly lower than that in group TA1, group TA4, group TA8 in comparison to the corresponding sub groups of group T（P0.05）; For the OD value of plasmic anti-cardiolipin antibodies（ACA）, no significant difference was observed during the corresponding sub groups of group N, group T and group TA（P0.05）. But the P-, E-selectin and ACA of pulmonary tissue in group TA was lowerly expressed by immunohistochemistry. Conclusion： Acute pulmonary embolism can lead to infiltration of inflammatory cell in rat lungs. The lung inflammation of acute pulmonary embolism rats can be enhanced probably by the increased release of P-, E-selectin and anticardiolipin antibodies, and the enhanced inflammation promotes the release of a series of inflammatory mediators, which exacerbate the injury of lung. Angelica injection relieves the lung inflammation of acute pulmonary embolism rats possibly by inhibiting the expression of P-, E-selectin and anti-cardiolipin antibody, thus playing a role in reducing thrombogenesis.