大鼠肢体缺血预处理对肝缺血/再灌注损伤的保护作用

Protective effect of limb ischemic preconditioning against liver ischemia/reperfusion injury in the rat

目的：研究肢体缺血预处理对大鼠肝缺血/再灌注损伤是否具有保护作用。方法：雄性SD大鼠32只,随机分为对照组（S组）;缺血/再灌注组（I/R组）;经典缺血预处理组（IPC组）;肢体缺血预处理组（远端缺血预处理组,RPC组）。S组仅行开腹,不作其他处理;IPC组以肝缺血5min作预处理;RPC组以双后肢缺血5min,反复3次作预处理,2个预处理组及I/R组均行肝缺血1h再灌注3h。取血用于血清谷丙转氨酶（ALT）与血清谷草转氨酶（AST）检测。切取肝组织用于测定湿干比（W/D）、中性粒细胞（PMN）计数及观察显微、超微结构的变化。结果：与I/R组比较,IPC组,RPC组ALT,AST,W/D值,及PMN计数均明显降低（P〈0.01）,肝脏的显微及超微结构损伤减轻。结论：肢体缺血预处理对大鼠肝脏I/R损伤有明显的保护作用,强度与经典缺血预处理相当,其机制可能与抑制肝脏炎症反应、减轻肝脏水肿、改善肝组织微循环有关。

Objective：To study the protective effect of limb ischemic preconditioning against liver ischemia/reperfusion injury in the rat. Methods： Rats were randomly divided into four groups （n=8）： ①Sham group （S group）, rats without ischemic preconditioning（IPC）, ②Ischemia/reperfusion（I/R） without IP （I/R group）; ③Rats with 5 min IPC （IPC group）; ④Rats with lower limbs IPC and repeated three times （remote ischemic preconditioning, RPC group）;The rats were subjected to 60-min sustained liver ischemia followed by 180-min reperfusion except S group.All ischemia rats were only subjected to 70% liver ischemia. Finally,blood and liver samples were obtained to determine the activity of ALT and AST, liver wet/dry weight （W/D） , PMN counts and pathology.Results： All IPC group and RPC group had obviously lower levels of ALT ,AST, W/D, PMN counts than that of the I/R group （P0.01）. Conclusion： The limb ischemic preconditioning has a protective effects against liver ischemia/reperfusion injury in the rat, possibly are due to suppression of liver inflammatory reaction, improvement of liver microcirculation.