紫外线A照射差异性损伤皮肤角质形成细胞和成纤维细胞

Differential Responses of Skin Keratinocytes and Fibroblasts to UVA-mediated Damage

紫外线A(ultraviolet A,UVA)照射皮肤可产生红斑、色素沉着以及光敏性损伤,导致光老化甚至诱导皮肤癌。研究发现,UVA(320～400 nm)照射对皮肤角质形成细胞和成纤维细胞造成损伤的反应和损伤程度都有所不同。本文结合近年来的相关研究,对UVA照射导致的上述两种细胞的形态、细胞膜损伤、血红素氧合酶(heme oxygenase,HO)活性、HO-1表达、细胞内有害自由铁形成,以及其它抗氧化物水平的不同反应等进行阐述,总结分析了同等剂量UVA照射后对角质形成细胞和成纤维细胞损伤的差异性,这些分析可能为皮肤抗UVA照射损伤以及寻找新型防晒剂的开发提供新思路和实验依据。

Ultraviolet A（UVA,320 ～400 nm） irradiation generates significant oxidative stress,leading to deleterious effects in human skin： erythema,pigmentation,photosensitivity,photoaging and skin cancer.Epidermal keratinocytes and dermal fibroblasts are known to have different responses to UVA irradiation.Compared to fibroblasts,keratinocytes appear to be far more resistant to UVA-mediated oxidative damage,and differ in the other responses.Here we review the most recent findings that elaborate the differences in UVA-mediated response in these two cell types,including cell morphology,membrane damage,heme oxygenase（HO） activity and HO-1 inducibility,levels of harmful ‘free’ labile iron and other antioxidants.These experimental evidences may provide new clues for improvement of therapy against UVA-induced skin damage and for development of novel sunscreens.