新型光敏剂分子二乙醇胺基竹红菌乙素光诱导HeLa细胞死亡中的氧化应激研究

Oxidative Stress Contributes to The Photocytotoxicity of EAHB as a Novel Photosensitizer to HeLa Cells

二乙醇胺基竹红菌乙素(2-ethanolamino-2-demethoxy-17-ethanolimino-hypocrellin B,EAHB)是一种新型的可吸收600 nm以上红光的竹红菌乙素衍生物。本文研究了二乙醇胺基竹红菌乙素-光动力诱导HeLa细胞死亡的效果及其氧化应激机制。结果发现,红光诱导后,MTT法检测到二乙醇胺基竹红菌乙素-光动力作用使HeLa细胞的存活率显著降低,且存活率与光敏剂浓度和光照剂量成反比;二乙醇胺基竹红菌乙素-光动力诱导HeLa细胞内产生活性氧自由基;同时,胞内超氧化物歧化酶和还原型谷胱甘肽水平显著降低,细胞脂质过氧化标志分子丙二醛显著升高,并检测到细胞质膜损伤标志分子乳酸脱氢酶的渗出显著增加。研究结果说明新型光敏剂二乙醇胺基竹红菌乙素可有效光诱导肿瘤细胞死亡,而细胞内氧化应激反应可能是二乙醇胺基竹红菌乙素光诱导肿瘤细胞死亡的重要作用机制。

2-ethanolamino-2-demethoxy-17-ethanolimino-hypocrellin B（EAHB）,a novel derivative of Hypocrellin B,was used here as a photosensitizer to probe its photocytotoxicity to the tumor cells and the intracellular oxidative stress.The survival rate of HeLa cells induced by EAHB mediated Photodynamic therapy（PDT） was decreased significantly as measured by MTT assay,and shown a negative dependence on both the concentration of photosensitizer and red light dose;Exposure to EAHB mediated PDT increased intracellular reactive oxygen species（ROS） levels and reduced intracellular glutathione levels.The increased production of malondialdehyde and lactate dehydrogenase release from the cells indicated lipid peroxidation and membrane damage.In summary,EAHB mediated PDT results in both the concentration of photosensitizer and the dose-dependent cytotoxicity in cultural HeLa cells that is closely correlated to increased oxidative stress.