密花石斛花粉的保存方法研究

Preservation of Dendrobium densiflorum pollen

研究了密花石斛花粉不同保存方法下的花粉寿命,并对密花石斛花粉的超低温保存技术程序进行了研究。结果表明:1)密花石斛花粉采用室温((27±2)℃)、冷藏(4℃)、冷冻(-20℃)保存,花粉寿命分别为5、23、17d,超低温保存(-196℃)的花粉120d后仍可检测到花粉萌发,且萌发率与新鲜花粉相同,超低温保存方法至少可以保存密花石斛花粉4个月;2)密花石斛花粉超低温保存的程序为新鲜花粉(含水量43.26%)直接投入液氮(LN)保存,需用时采用室温、自来水或水浴化冻即可。

This paper focuses on the effects of different storage temperatures on the pollen longevity of Dendrobium densiflorum, and provides one promising cryopreservation procedure. Results indicated that： 1 ） The pollen longevity at （27 ±2） ℃ was only 5 days, 23 days at 4℃ and 17 days at -20℃ , while the pollen storaged in liquid nitrogen （LN） （ - 196℃ ） can also germinate even after 120 days, and the germination level of which was comparable with that of the fresh pollen. These data showed that cryopreservation was suitable for pollen preservation of D. densiflorum in a short or midium term. 2） The optimum cryopreservation procedure was immersing fresh pollen （moisture content 43.26% ） into LN without desiccation, and thawing at room temperature by running water or warm water （37℃） after cryopreservation.