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一种胚胎体外培养的新方法:滴中孔培养法 被引量：1

A New Method for Embryo Culture in vitro: the Well in the Drop (WID) System
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摘要 目的:建立并优化一种胚胎体外培养的新方法——滴中孔培养法。方法:用圆头玻璃管在培养皿上烫出多个圆孔,作为滴中孔培养的专用平皿;收集牛卵丘-卵母细胞复合体(COCs)并在滴中孔培养专用平皿中进行体外成熟(IVM)、体外受精(IVF)和体外培养(IVC)。结果:①滴中孔培养法与群卵共培养的效果相似,而单卵培养明显降低了卵母细胞的发育能力(P<0.05)。②培养滴体积和培养密度对卵母细胞核成熟没有明显的影响,但显著影响胚胎的发育能力。在培养密度不变的情况下,培养滴体积太小显著削弱了胚胎的发育能力,而培养密度过小或者过大也削弱胚胎的发育能力(P<0.05)。结论:滴中孔培养法可以达到群卵共培养的效果。 Objective： To establish and optimize a new method of embryo culture in vitro： the well in the drop （WID） system. Methods： The glass tube was used to make some round hole on the dish, to make a special dish for WID system; Bovine COCs and cultured in WID system in vitro maturation （IVM）, in vitro fertilization （IVF） and in vitro culture （IVC）. Results： 1） The WID culture system works as efficient as group culture, whereas the individual culture impaired the oocyte developmental competence significantly （P〈0.05）. 2） The medium volume and density had no effect on oocyte nuclear maturation but affect embryonic development significantly. As to development in vitro, it was proved that too small medium volume was detrimental to embryo development with the same culture density. Besides, too low or too high culture density was proved detrimental to embryo development （P〈0.05）. Conclusion： The WID culture system can achieve the effect of group culture.
作者 冯卫国 潘智芳 刘晓影 于文静 赵春玲 高志芹
机构地区 潍坊医学院
出处 《生殖与避孕》 CAS CSCD 北大核心 2010年第11期721-725,共5页 Reproduction and Contraception
基金 山东省自然科学基金资助项目(ZR2009DL013)
关键词 滴中孔培养法 培养滴体积 培养密度 卵母细胞发育能力 牛 WID medium volume culture density oocyte developmental competence bovine
分类号 Q813.7 [生物学—生物工程]
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参考文献22

  • 1Kato Y, Tsunoda Y. Effects of the culture density of mouse zygotes on the development in vitro and in vivo.Theriogenology, 1994, 41(6): 1315-22.
  • 2Ferry L, Mermillod P, Massip A, et al. Bovine embryos cultured in serum-poor oviduct-conditioned medium need cooperation to reach the blastocyst stage. Theriogenology, 1994, 42(3):445-53.
  • 3Moessner J, Dodson WC. The quality of human embryo growth is improved when embryos are cultured in groups rather than separately. Fertil Steril, 1995, 64(5): 1034-5.
  • 4Nagao Y, Saeki K, Nagai M. Effects of embryo density, oxygen concentration and medium composition on in vitro development of bovine early embryos. Theriogenology, 1998, 49(3):210-2.
  • 5Palasz AT, Thundathil J. The effect of volume of culture medium and embryo density on in vitro development of bovine embryos. Theriogenology, 1998, 49(3):212-5.
  • 6Hendriksen PJM, Bevers MM, Dieleman S. Single IVP using BRL cell co-cuture and serum yields a lower blastocyst rate than group culture. Theriogenology, 1999, 51 (4):319-21.
  • 7Jewgenow K, Heerdegen B, Muller K. In vitro development of individually matured bovine oocytes in relation to follicular wall atresia. Theriogenology, 1999, 51(4): 745-56.
  • 8O'Doherty EM, Wade MG, Hill JL, et al. Effects of culturing bovine oocytes either singly or in groups on development to blastocysts. Theriogenology, 1997, 48 (8):161-9.
  • 9Donnay I, van Langendonckt A, Auquier P, et al, Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology, 1997, 47(8): 1549-61.
  • 10Fukui Y, Kikuchi Y, Kondo H, et al. Fertilizablility and developmental capacity of individually cultured bovine oocytes. Theriogenology, 2000, 53(8):1553-65.

二级参考文献15

  • 1黄娟华,刘炜培,张羽虹,李颖嫦.人早期胚胎卵裂球全能性的体外实验研究[J].中国优生与遗传杂志,2006,14(2):89-90. 被引量：4
  • 2Mitalipova M, Calhoun J, Shill S, et al. Human embryonic stem cell lines derived from discarded embryos. Stem Cells, 2003, 21(5):521-6.
  • 3Chen H, Qian K, Hu J, et al. The derivation of two additional human embryonic stem cell lines from day 3 embryos with low morphological scores. Hum Reprod, 2005, 20 (8):2201-6.
  • 4Tarkowski, Andrzej K. Experiments on the development of isolated blastomeres of mouse eggs. Nature, 1959, 184 (4695):1286-7.
  • 5Klimanskaya I, Chung Y, Becker S, et al. Human embryonic stem cell lines derived from single blastomeres. Nature, 2006, 444(7118):481-5.
  • 6Matsumoto K, Miyake M, Utsumi K, et al. Production of identical twins by separating two-cell rat embryos. Gamete Res, 1989, 22(2):257-63.
  • 7Johnson WH, Loskutoff NM, Plante Y, et al. Production of four identical calves by the separation of blastomeres from an in vitro derived four cell embryo. Vet Rec, 1995, 137(1):15-7.
  • 8Eckert J, Tao T, Niemann H. Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8-cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human leukemia inhibitory factor. Biol Reprod, 1997, 57(3):552-60.
  • 9van de Velde H, Cauffman G, Tournaye H, et al. The four blastomeres of a 4-cell stage human embryo are able to develop individually into blastocysts with inner cell mass and trophectoderm. Hum Reprod, 2008, 6(1):1-6.
  • 10Feki A, Jean-Bernard Dubuisson. Derivation of the first Swiss human embryonic stem cell line from a single blastomere of an arrested four-cell-stage embryo. Swiss Med Wkly, 2008, 138(1):37-8.

共引文献6

同被引文献22

  • 1Rijnders PM, Jansen CAM. Influence of group culture and culture volume on the formation of human blastocycsts: a prospective randomized study. Hum Reprod, 1999, 14(9): 2333-7.
  • 2Spyropoulou I, Karamle C, Bolton VN. A prospective ran- domized study comparing the outcome of in-vitro fertiliza- tion and embryo transfer following culture of human em- bryos individually or in groups before embryo transfer on day 2. Hum Reprod, 1999, 14(1):76-9.
  • 3Ge HS, Huang XF, 2/rang W, et al. Exposure to human chorionic gonadotropin during in vitro maturation does not improve the maturation rate and developmental potential of immature oo- cytes from patients with polycystic ovary syndrome. Fertil Steril, 2008, 89(1):98-103.
  • 4O'Doherty EM, Wade MG, Hill JL, et al. Effects of culturing bovine oocytes either singly or in groups on development toblastocysts. Theriogenology, 1997: 48(1): 161-9.
  • 5Gardnar DK, Lana M, Spitzer A, et al. Enhanced rates of cleavaged and development for sheep zygotes cultured to theblastocyst stage in the absence serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulate development. Biol Reprod, 1994, 50(2):390-400.
  • 6Stoddart NR, Wild AE, Fleming TP. Stimulation of develop- ment in vitro by platelet-activating factor receptor ligands released by mouse preimplantation embryos. J Reprod Fertil, 1996, 108(1):47-53.
  • 7Rebollar-Lazaro I, Matson P. The culture of human cleavage stage embryos alone or in groups: effect upon blastocyst utilization rates and implantation. Biol Reprod, 2010, 10(3): 227-34.
  • 8Moessner J, Dodson WC. The quality of human embryo growth is improved when embryos are cultured in groups rather than separately. Fertil Steril, 1995, 64(5): 1034-5.
  • 9Tao T, Robichaud A, Mercier J, et al. Influence of group embryo culture strategies on the blastocyst development and pregnancy outcome. J Assist Reprod Genet, 2012,12 (14):63-8.
  • 10Kate K, Tsunoda Y. Effects of the culture density of mouse zygotes on the development in vitro and in vivo. Therio- genology, 1994, 41(6):1315-22.

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生殖与避孕
2010年 第11期

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