摘要
目的:探讨T细胞淋巴瘤侵袭转移诱导因子l(Tiam1)在动情周期中小鼠子宫内膜的表达规律。方法:采用RT-PCR、免疫组织化学法和Western blotting分别检测小鼠子宫内膜在动情前期、动情期、动情后期和动情间期Tiam1mRNA和蛋白的表达规律。结果:在动情周期中,小鼠子宫内膜mRNA在动情期表达最高,与其它3期相比具有显著性差异,P<0.01;免疫组织化学和Westernblotting的结果也显示Tiam1蛋白在动情期子宫内膜的表达规律与RT-PCR的结果一致。结论:Tiam1在小鼠动情周期子宫内膜中呈动态表达,提示Tiam1可能参与了动情周期子宫内膜变化的调控。
Objective: To investigate the spatio-temporal expression profile of T-lymphoma invasion and metastasis inducing protein 1 (Tiam1) in endometria during the estrus cycle. Methods: RT-PCR, immunohis- tochemistry techniques and Western blotting were used to test the expression of Tiam1 mRNA and protein in endometria during the estrus cycle, respectively. Results: The expression of both Tiam1 mRNA and its protein was higher in mice endometrium at estrus than that of the other 3 stages (P〈0.01). The expression pattern of Tiam1 protein was similar to its mRNA. Conclusion: Tiam1, with regular expression in the estrus cycle, might play an important role in the endometrial cyclical variation during the estrus cycle.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2010年第11期726-731,共6页
Reproduction and Contraception