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Oligodendrocyte transcription factor 1 mRNA and protein expression in organotypic rat brain slices

Oligodendrocyte transcription factor 1 mRNA and protein expression in organotypic rat brain slices
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摘要 Numerous studies have confirmed that oligodendrocyte transcription factor 1 （Olig-1） is vital for myelin repair. However, the effects of hypoxia and ischemia on Olig-1 expression remain unknown. In this study, Olig-1 mRNA and protein expressions were analyzed by in situ hybridization and immunohistochemistry, to determine the expression profile of Olig-1 in rat brain slices exposed to hypoxia and ischemia. Brains were obtained from 2-day-old Sprague-Dawley rats, and sections were randomly assigned to control and hypoxia/ischemia groups. Hematoxylin-eosin staining revealed karyorrhexis and karyopyknosis in cells from the hypoxia/ischemia group. Under electron microscopy, mitochondria swelling and neuropil edema were observed in the hypoxiaJischemia group. Olig-1 mRNA and protein expressions were increased at 1 day after hypoxia and ischemia treatment. These results suggest that in situ hybridization and immunohistochemistry could be used simultaneously to detect mRNA and protein expression in brain slices. Numerous studies have confirmed that oligodendrocyte transcription factor 1 （Olig-1） is vital for myelin repair. However, the effects of hypoxia and ischemia on Olig-1 expression remain unknown. In this study, Olig-1 mRNA and protein expressions were analyzed by in situ hybridization and immunohistochemistry, to determine the expression profile of Olig-1 in rat brain slices exposed to hypoxia and ischemia. Brains were obtained from 2-day-old Sprague-Dawley rats, and sections were randomly assigned to control and hypoxia/ischemia groups. Hematoxylin-eosin staining revealed karyorrhexis and karyopyknosis in cells from the hypoxia/ischemia group. Under electron microscopy, mitochondria swelling and neuropil edema were observed in the hypoxiaJischemia group. Olig-1 mRNA and protein expressions were increased at 1 day after hypoxia and ischemia treatment. These results suggest that in situ hybridization and immunohistochemistry could be used simultaneously to detect mRNA and protein expression in brain slices.
作者 Hong Cui Lijun Yang Dezhuang Huang Wandong Zhang Weijuan Han Yanqing Yao Wenxing Jiang
机构地区 Pediatrics Department Hepatitis Institute Institute for Biological Sciences
出处 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第21期1639-1643,共5页 中国神经再生研究（英文版）
基金 the National Natural Science Foundation of China, No. 30872778 the Natural Science Foundation of Beijing City, No. 7072023 Clinical Basic Corporation Foundation of Capital Medi-cal University, No. 2006jl18
关键词 oligodendrocyte transcription factor 1 double staining brain slices immunohistochemistry in situ hybridization hypoxia and ischemia oligodendrocyte transcription factor 1 double staining brain slices immunohistochemistry in situ hybridization hypoxia and ischemia
分类号 Q2 [生物学—细胞生物学] Q78 [生物学—分子生物学]
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Neural Regeneration Research
2010年 第21期

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