纳米羟基磷灰石对人脑胶质母细胞瘤U87细胞活性抑制效应的研究(英文)

Inhibitory Effect of Nano-HA Particles on Human U87 Glioblastoma Cells Viability

为了评价不同形貌的纳米羟基磷灰石粒子(nano-HA)对脑胶质母瘤细胞系U87细胞的作用,合成了三种不同形貌的nano-HA,与U87细胞共培养,培养基中同时还添加了聚乙二醇(PEG),以保持nano-HA的稳定性.透射电镜(TEM)及动态光学散射(DLS)测定高度分散在培养基中nano-HA的稳定情况和粒子大小,MTT法检测了nano-HA对U87细胞的作用.实验结果表明,nano-HA可显著抑制U87细胞增殖;并且,针状纳米粒子的抑制能力要强于另外两种粒子.此外,通过RT-PCR检测发现不同形貌的nano-HA可不同程度的下调细胞的Toll样受体4(TLR4)的表达水平,这可能是其具有不同生物学活性的原因之一.

To evaluate the effects of Hydroxyapatite nano-particles（nano-HA） with different morphologies on human U87 glioblastoma cell line（U87）,three kinds of nano-HA were designed and synthesized,and a co-culture system containing nano-HA and U87 cells was established.Polyethylene glycol（PEG） was also added to the HA medium system for the samples stabilization.After determining the stabilization and high dispersed nanometer size of nano-HA in the medium system using Transmission electron microscope（TEM） and dynamic light scattering（DLS）,the effects of nano-HA on GBM cells were assessed with an MTT method.Results showed that nano-HA could effectively inhibit U87 cells proliferation.Compared with other nano-HA,the needle-like nano-HA were more powerful in inhibiting U87 cells proliferation.Toll-like receptor 4（TLR4） induced by different nano-HA may be one reason for their different bioactivity.