小针高频电凝损伤鸡冠毛细血管的初步研究

Effect of high frequency electrocoagulation by ultrafine-needle on the cutaneous capillary hemangiomas：a preliminary study on cock combs

目的 以鸡冠作为皮肤毛细血管瘤的动物模型,观察不同功率小针高频电凝治疗后鸡冠的病理学变化,探讨该法损伤鸡冠毛细血管的功率选择. 方法 32只雄性莱亨鸡随机分成4组,每组8只动物：1个空白对照组和3个实验组,实验各组在相同条件下,分别给予不同功率的小针高频电凝,即6 W组、8 W组和10 W组.观察鸡冠外观,并从电凝后即刻、术后7、14、21、28 d 5个时间点切取鸡冠组织进行病理学观察. 结果 鸡冠经小针高频电凝后,色泽变白、瘢痕形成,功率越大鸡冠外形改变越明显.光镜下,电凝即刻,电凝各组毛细血管数量均明显少于空白对照组（P＜0.05）.随时间的推移毛细血管有一定的增生,7 d以后各实验组间毛细血管数量差异无统计学意义（P＞0.05）,28 d电凝各组毛细血管数量仍明显少于空白对照组（P＜0.01）.电凝后修复过程中,鸡冠组织胶原纤维合成,电凝各组胶原纤维合成明显多于空白对照组（P＜0.05）,但6 W组胶原纤维合成少于8 W和10 W组.胶原纤维合成与电凝功率呈正相关关系（P＜0.05,相关系数r＞0.5）. 结论 在前述实验条件下,使用6 W的高频电凝功率小针高频电凝能有效减少鸡冠毛细血管数量,且不造成鸡冠外形改变,符合微创、美容原则,提示临床上应用该技术治疗毛细血管瘤可能具有可行性.

Objective To investigate the injury effect of high frequency electrocoagulation（HFE）by ultrafine-needle on the cutaneous capillary hemangiomas by hispathologic study of cock comb. Methods 32 male cocks were randomly divided into 4 groups with 8 animals in each group. 3 experimental groups received HFE with different power （6W, 8W, 10W） on the cock comb. Specimens were taken from cock combs for histologic study immediately after treatment and 7, 14, 21, 28 days later. Results After teatment, the cock combs turned pale and followed with scar formation. The shape of combs changed a lot after high power treatment. Immediately after treatment, the capillary number in the experimental groups decreased markedly under light microscope, compared with that in control group（ P〈0. 05 =. After that,the capillary number in experimental group increased. 7 days after treatment, the capillary number in experimental groups was still significantly lower than that in control groups （P〈0. 01 =, but not markedly different between the three experiment groups（ P〉0.05 ）. Collagen formation also increased after treatment （ P〈0. 05 =, which was higher in experimental groups with high power（ P〈0. 05, correlation coefficient r〉0. 5=. Conclusions HFE with ultrafine needle can effectively decrease the capillary number in cock comb. The 6W treatment is mini-invasive and will not destroy the comb shape with good cosmetic results.The technique may be used for the treatment of cutaneous capillary hemangioma.