核因子кB受体激动子配体和骨保护因子mRNA在慢性根尖周炎病损组织中的表达

Expression of RANKL and OPG mRNA in apical periodontitis

目的:探讨核因子кB受体激动子配体(receptor activator of nuclear factor κB ligand,RANKL)和骨保护因子(osteoprotegerin,OPG)在慢性根尖周炎发病中的作用。方法:采用RT-PCR法,从mRNA水平检测RANKL、OPG在10例慢性根尖周肉芽肿和3例根尖周囊肿中的表达。结果:RANKL mRNA在根尖周肉芽肿病损组和根尖周囊肿组明显增高,与正常对照组相比差异均有统计学意义(P<0.05),但根尖周肉芽肿和根尖囊肿组之间差异无显著性(P>0.05)。OPG mRNA仅在两个病损组织中检测到有低水平的表达,其余各病损组织和正常的牙周膜组织中均未检测到OPG mRNA的表达。结论:RANKL和OPG在慢性根尖周炎的发病中具有重要的作用,但也提示存在其他细胞因子的协同作用。

AIM：To explore the role of receptor activator of nuclear factor κB ligand （ RANKL） and osteoprotogerin （ OPG） in the pathogenesis and development of periapical lesions. METHODS：Ten periapical granulomas and 3 periapical cysts were obtained. The expression of RANKL and OPG mRNA was detected by reverse transcription polymerase chain reaction （ RT-PCR） technique. RESULTS：A significant higher level of RANKL mRNA was found in periapical granulomas and periapical cysts. Low level of OPG mRNA was detected in only two periapical granuloma samples,but was not detected in other lesions and normal controls. CONCLUSION：RANKL and OPG might play important roles in the pathogenesis and development of periapical lesions.