香蕉BTB/POZ域基因的克隆及表达分析

Cloning and Expression Analysis of BTB/POZ Gene in Banana

利用抑制缩减杂交文库从香蕉中分离获得一个cDNA片段,结合RACE技术获得该基因的全长序列,命名为MaBTB基因,该cDNA的ORF全长1080个碱基。通过Blastx同源性分析结果显示该基因的氨基酸序列与水稻OsBTB、苜蓿MtBTB/POZ、拟南芥AtBPM4、葡萄VvBTB、玉米ZmBTB、松树PsBTB基因的氨基酸序列同源性很高,分别为86%、85%、84%、84%、86%和87%。遗传进化树分析发现该基因与苜蓿MtBTB/POZ和拟南芥AtBPM4基因亲缘关系较近。用RT-PCR的方法研究该基因在不同胁迫处理下的表达特性,结果表明该基因在盐、乙烯和紫外线处理后其表达量逐渐增强;低温处理和香蕉尖孢镰刀菌4号生理小种处理后该基因表达量开始降低,随后逐渐升高;伤害处理后该基因的表达量没有明显变化。

A full length cDNA named MaBTB（BR-C,ttk and bab or pox virus and zinc finger）,which contained 1 080bp with an open reading frame,was isolated by SSH combined with RACE technology.Sequence analysis revealed that this gene shared high identities of 86%、85%、84%、84%、86% and 87% to those of BTB/POZ from Oryza sativa（OsBTB）,Medicago truncatula（MtBTB/POZ）,Arabidopsis thaliana（AtBPM4）,Vitis vinifera（VvBTB）,Zea mays（ZmBTB）and Picea sitchensis（PsBTB））,respectively.Phylogenetic analysis showed that MaBTB was closely related to Medicago truncatula（MtBTB）and Arabidopsis thaliana（AtBPM4）.The expression feature of MaBTB was investigated by RT-PCR.The result showed that under salt,ethylene and ultraviolet radiation treatment,the expression of MaBTB gradually increased.The accumulation of MaBTB decreased and then gradually increased with cold and FOC race 4 treatment.However,the accumulation of MaBTB was not distinct variety by wounding treatment.