摘要
利用抑制缩减杂交文库从香蕉中分离获得一个cDNA片段,结合RACE技术获得该基因的全长序列,命名为MaBTB基因,该cDNA的ORF全长1080个碱基。通过Blastx同源性分析结果显示该基因的氨基酸序列与水稻OsBTB、苜蓿MtBTB/POZ、拟南芥AtBPM4、葡萄VvBTB、玉米ZmBTB、松树PsBTB基因的氨基酸序列同源性很高,分别为86%、85%、84%、84%、86%和87%。遗传进化树分析发现该基因与苜蓿MtBTB/POZ和拟南芥AtBPM4基因亲缘关系较近。用RT-PCR的方法研究该基因在不同胁迫处理下的表达特性,结果表明该基因在盐、乙烯和紫外线处理后其表达量逐渐增强;低温处理和香蕉尖孢镰刀菌4号生理小种处理后该基因表达量开始降低,随后逐渐升高;伤害处理后该基因的表达量没有明显变化。
A full length cDNA named MaBTB(BR-C,ttk and bab or pox virus and zinc finger),which contained 1 080bp with an open reading frame,was isolated by SSH combined with RACE technology.Sequence analysis revealed that this gene shared high identities of 86%、85%、84%、84%、86% and 87% to those of BTB/POZ from Oryza sativa(OsBTB),Medicago truncatula(MtBTB/POZ),Arabidopsis thaliana(AtBPM4),Vitis vinifera(VvBTB),Zea mays(ZmBTB)and Picea sitchensis(PsBTB)),respectively.Phylogenetic analysis showed that MaBTB was closely related to Medicago truncatula(MtBTB)and Arabidopsis thaliana(AtBPM4).The expression feature of MaBTB was investigated by RT-PCR.The result showed that under salt,ethylene and ultraviolet radiation treatment,the expression of MaBTB gradually increased.The accumulation of MaBTB decreased and then gradually increased with cold and FOC race 4 treatment.However,the accumulation of MaBTB was not distinct variety by wounding treatment.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2010年第11期30-33,共4页
China Biotechnology
基金
中央级公益性科研院所基本科研业务专项(ITBBZD1031)
公益性行业(农业)科研专项(nyzx07-029)
现代农业产业技术体系建设专项资金(nycytx-33)资助项目