摘要
实验选取鸡粪和猪粪进行好氧堆肥发酵,研究畜禽粪便腐熟过程中酶活性和微生物的变化趋势以及相互联系。结果表明:过氧化氢酶活性和纤维素酶活性在堆肥初期较高,随后迅速降低,最终过氧化氢酶维持在9~12ml/g之间,纤维素酶维持在12.37~15.07mg/(kg.h)之间,而脲酶活性变化趋势为"升高-降低-升高"。细菌数量变化趋势为"低-高-低";放线菌为"高-低";真菌为"高-低-高"。通过相关分析发现,放线菌可能是影响堆肥中过氧化氢酶和纤维素酶的关键因素。鸡粪中放线菌与过氧化氢酶呈极显著正相关;猪粪中放线菌与过氧化氢酶和纤维素酶呈显著正相关;鸡粪+猪粪中放线菌与过氧化氢酶和纤维素酶呈极显著正相关。
The experiment took chicken manure and pig manure to conduct composting experiment and researched on changes and interrelationships of enzyme activity and microbe quantity.The results showed that:the catalase activity and cellulose activity kept at a higher level in the early stage of composting,and then decreased quickly.Finally,the catalase activity between 9ml/g and 12ml/g,and the cellulose activity between 12.37mg/(kg·h)and 15.07mg/(kg·h).The trend of urease activity was "highed-lowed-highed".The trend of bacteria quantity was "low-high-low",actinomycetes quantity was "high-low" and fungi was "high-low-high".Through correlation analysis,the actinomycetes may was important factor that affected the change of catalase activity and cellulose activity.The actinomycetes had a significantly positive correlation with catalase in chicken manure,and had a positive correlation with catalase and cellulose in pig manure,and had a significantly positive correlation with catalase and cellulose in mixed chicken and pig manure.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2010年第11期56-60,共5页
China Biotechnology
基金
国家自然科学基金(40971155)
广东省教育部产学研结合项目(2009B090300330)资助项目
关键词
畜禽粪便
堆肥
酶活性
微生物数量
Animal manure Composting Enzyme activity Microbe quantity