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HULC RNA在肝癌细胞中的表达及其对邻近基因SLC35B3表达的影响 被引量：10

Specifically up-regulated non-coding RNA gene HULC in tumor cell lines and its effects on the expression of neighboring gene SLC35B3
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摘要 目的 探讨长片段非编码RNA--肝癌高表达基因（HULC）RNA在不同的肿瘤细胞系中的表达情况以及对其邻近基因表达的影响.方法 应用反转录-聚合酶链反应（RT-PCR）方法 在不同来源的肿瘤细胞系中检测HULC RNA的表达情况,并通过生物信息学的方法 寻找与其邻近的基因,再通过实时PCR技术检测HULC对邻近基因的表达情况的影响.结果 在检测的8株细胞系：人胚肾细胞系293T,食管癌细胞系EC9706、KYSE150,结肠癌细胞系HCT116,肝癌细胞系SMMC7721、HepG2以及肺癌细胞系H446、H1229中,非编码RNA HULC在HepG2细胞中的表达量特异性升高.HULC基因的附近（＜1000 bp）没有编码基因存在,与其距离最近的基因是SLC35B3,且间距在约200 000 bp.与HULC RNA表达量较低的SMMC-7721细胞相比,内源性高表达HULC RNA的HepG2细胞中SLC35B3的转录更高[（0.477±0.040）%比（0.129±0.004）%,P＜0.01].在SMMC7721细胞中转染pcDNA3.1-HULC质粒后,其邻近基因SLC35B3表达略微升高.而通过RNA干扰的方法 敲降HULC RNA后,SLC35B3基因的表达也随之下降.HepG2细胞转染2条小干扰RNA（siRNA）后,SLC35B3基因的表达分别为[（0.283±0.007）%和（0.387±0.015）%],并且第1条siRNA转染后与阴性对照[（0.477±0.042）%]比较差异有统计学意义（P＜0.01）.结论 肝癌细胞HepG2中特异性高表达的非编码RNA HULC对其邻近基因SLC35B3的表达有一定影响作用. Objective To study the expression of non-coding RNA HULC in different rumor cell lines and its effects on the expression of neighboring genes. Methods The expression levels of HULC were detected in different tumor cell lines. And the neighboring genes of HULC were searched by bioinformatics.Then its effects on the neighboring genes were examined by real-time polymerase chain reaction （PCR）.Results The expression levels of HULC RNA were detected in the 293T, EC9706, KYSE150, HCT116,SMMC-7721, HepG2, H446 and H1299 cell lines. Non-coding RNA HULC was specifically highly upregulated in hepatocarcinoma cell line HepG2. Little coding genes existed around HULC gene （ ＜ 1000 bp） and the nearest coding gene was SLC35B3 at 200 000 bp away from the location of HULC gene. The SMMC-7721 cell line had a low endogenous expression of HULC gene. In comparison with SMMC-7721 cell line, the expression of SLC35B3 gene in HepG2 cell line was higher [ （0. 477 ± 0. 040） % vs （0. 129 ± 0. 004） %, P ＜0. 01 ]. The exogenous expression of HULC had little effects on SLC35B3 gene. But knocking down the expression of HULC gene by siRNA attenuated the expression of SLC35B3 [ siA （0. 283 ± 0. 007 ）%, siB （0.387±0.015）%,control（0.477 ±0.042）%]. Conclusion The specifically up-regulated non-coding gene HULC in HepG2 cell line has some effects on the expression of its neighboring gene SLC35B3.
作者 李丹 宋咏梅 詹启敏
机构地区 中国医学科学院北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室
出处 《中华医学杂志》 CAS CSCD 北大核心 2010年第44期3156-3159,共4页 National Medical Journal of China
基金 国家重点基础研究发展计划＂973＂项目基金（2009CB521801）
关键词 RNA 未翻译 计算生物学 肝癌高表达基因RNA 基因 SLC35B3 RNA,untranslated Computational biology HULC RNA Gene, SLC35B3
分类号 R686 [医药卫生—骨科学]
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参考文献13

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同被引文献108

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引证文献10

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中华医学杂志
2010年 第44期

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