摘要
生物学研究及种类鉴定是绿潮研究的热点和难点之一。本文系统阐述了石莼科绿藻常见种类的生物学特征,探讨了系统分类研究难点,并利用ITSr、bcL等基因序列以及质体蓝素氨基酸序列进一步研究分析了石莼科10种绿藻的序列结构特征和分子系统学关系。研究显示,石莼科浒苔属和石莼属绿藻rbcL和ITS序列差异未体现出属间分化的差异,根据rbcL序列和ITS序列分别构建的系统进化树中,石莼和孔石莼、肠浒苔、扁浒苔都聚类到同一进化枝上,而裂片石莼和网石莼、浒苔和缘管浒苔聚类到另一进化枝上,这显示出在分子进化水平上较为一致的结果,为浒苔属和石莼属系统分类深入研究提供一定的证据;根据质体蓝素所构建的系统进化树中,阿氏石莼和孔石莼在NCBI上发表的质体蓝素氨基酸序列在第1进化枝中,浒苔的质体蓝素氨基酸序列在第2进化枝中。但在第1进化枝中,孔石莼的3个质体蓝素氨基酸序列没有完全分布在同一个分支上。
Biological research and species identification is one of the hotspots and difficulties of green tides study.This paper describes the biological characteristics of common green algae of Ulvaceae and discusses the difficulties of systemic classification.ITS sequences,rbcL sequences and plastocyanin amino acid sequences were used to analyze the molecular systematics of 10 green algae of Ulvaceae.Studies showed that rbcL and ITS sequences between Ulva and Enteromorpha didnot reflect intergeneric differentiation.According to the phylogentic trees constructed respectively by rbcL sequences and ITS sequences,Ulva lactuca,Ulva pertusa,Enteromorpha intestinalis and Enteromorpha compressa were clustered to the same evolutionary branch and Ulva fasciata,Ulva reticulata,Enteromorpha prolifera and Enteromorpha linza were clustered to another,indicating consistent results at the molecular evolutionary level.It also provided evidence for further research between Ulva and Enteromorpha.According to the phylogentic tree constructed by plastocyanin amino acid sequences,the sequence of Ulva arasakii and the sequences of Ulva pertusa were clustered to the first evolutionary branch and the sequences of Enteromorpha prolifera were clustered to the second.Three sequences of Ulva pertusa were clustered to different evolutionary branches.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第12期71-80,共10页
Periodical of Ocean University of China
基金
青岛市科技项目(09-2-5-5-hy)
国家科技支撑计划项目(2008BAC49B01)资助
