用中子活化分析法研究25种元素在人体肝脏亚细胞组分中的分布

Subcellular Distribution of 25 Elements in the Human Liver by Neutron Activation Analysis

采用差速离心分离技术与中子活化分析法(NAA)相结合的方法分析了人肝25种元素,并用标准参考物保证结果的准确性.分析结果表明,大多数元素在各亚细胞组分中并非均匀分布.As、Au、Co、I、Mg、Mo、Sb、Sc在细胞核和线粒体浓度最高.Al、As、Au、Ba、Fe、I、La、Mg、Mn、Sb、Se在胞液中浓度最低,生物体内主要以离子形式存在的Br、Cl、K、Na则在胞液中浓度最高.值得注意的是,As在细胞核中大量聚集,是其它组分的2～4倍;而La与Fe则在微粒体中富集.

The distribution pattern of 25 elements in subcellular fractions of human liver was studied by differential centrifugation combined with neutron activation analysis. The analytical quality had been verified by the analysis of some standard reference materials. The highest concentrations of As, Au, I, Mg, Mo, Sb, Sc, Se were found in nuclei and mitochondria, whereas those of Br, Cu, Cl, K and Na in cytosol. The lowest concentrations of Al, As, Au, Ba, Fe, I, La, Mg, Mn, Sb, Se were found in cytosol. Interestingly, As was mainly present in the nuclear fraction and was two to four times than that in the other fractions, while La and Fe were enriched in the microsomal fraction.