应用激光解吸质谱和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析蛇毒精氨酸酯酶的纯度和分子量

Application of Matrix-assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis in Determining Arginine Esterase From Snake Venom

应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)和SDS-聚丙烯酰胺凝胶电泳对吉林省两地市的同种白眉蝮蛇蛇毒中具有抗栓塞药效的精氨酸酯酶进行了分析和比较。MALDI-TOF-MS法具有快速、准确度高、灵敏度高的优点,两种方法结合,互为补充,取得了令人满意的结果,MALDI-TOF-MS完全可以直接用作蛇毒成分分离过程中重要的研究手段。

Using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) . The homogeneities and molecular weights of three arginine esterases from snake venom, which possessing therapeutic use in myocardial infartion, were determined and compared, MALDI-TOF-MS is possessed of high accuracy, high sensitivity and rapidity. MALDI-TOF-MS and sodium dodecyl sulfat-polyacrylamide gel electrophoresis (SDS-PAGE) can provide complementary and confirmatory results information. MALDI-TOF-MS can be directly used as an important method for the purification of snake venom complexes successfully.