吸水链霉菌BS-112菌株产生抗家蚕病原真菌活性物质的发酵培养基配方优化

Optimization of Medium Composition for the Production of Antifungal Active Substances from Streptomyces hygroscopicus BS-112 Against Silkworm Pathogenic Fungi

为了开发利用吸水链霉菌BS-112菌株产生的抗真菌活性物质防治家蚕真菌病,在单因素试验基础上通过正交试验优化BS-112菌株分泌产生抗真菌活性物质的发酵培养基配方。优化后的最佳发酵培养基配方为:18.0g/L葡萄糖,12.0g/L玉米粉,35.0g/L黄豆饼粉,0.4g/LKH2PO4,0.8g/LMgSO4。优化发酵培养基配方后的菌株发酵液对家蚕病原绿僵菌的抗菌效价达到4916.85μg/mL,较基础培养基提高了3.08倍。分别以价格低廉的玉米粉和豆饼粉作为发酵培养基的缓效碳源和有机氮源,有利于该菌株在蚕用抗生素产业化开发的应用。

To develop the biological control agent against silkworm pathogenic fungi through the antifungal active substances produced by Streptomyces hygroscopicus BS-112,the fermentation medium for production of the active substance was optimized through orthogonal design based on single factor tests.The optimal medium for the production of antifungal active substances had the following composition：glucose 18.0 g/L,corn meal 12.0 g/L,soybean powder 35.0 g/L,KH2PO4 0.4 g/L,and MgSO4 0.8 g/L.The fermentation liquid from optimized medium had an antifungal potency against silkworm green muscardine pathogen of 4 916.85 μg/mL,being 3.08 times higher compared to the yield of active substances that were produced using the basal medium.The low-cost corn meal and soybean powder were used as slow-release carbon and organic nitrogen sources for the fermentation medium respectively,being beneficial for the application of BS-112 strain in industrial development of silkworm antibiotics.