摘要
合成了新荧光试剂水杨醛-2-羧基苯腙(SACPH)。研究了试剂与铝的荧光反应新体系的最适宜条件。在pH=4.0的乙醇/水(3.5:6.5,V/V)溶液中,该试剂与铝离子形成稳定的络合物,组成比为1:1(金属∶试剂),λex/em=316.8/456.0 nm。所建立铝的荧光分析方法的线性范围为0.5～120.0μg/L,检出限为0.20μg/L,方法成功的应用于油条、牛奶、啤酒、杏仁露中铝的测定。
A new fluorescent reagent,salicyladehyde-2-carboxy-phenylhydrazone（SACPH） was synthesized and the fluorescent reaction of this reagent with aluminium ions was studied in detail.Based on this chelation reaction,a spectrofluorimetric method was developed for the determination of aluminium in ethanol-water（3.5∶6.5,V/V） medium at pH 4.0.Under these conditions the Al3＋-SACPH complex has excitation and emission maxima at 316.8nm and 456.0nm respectively.The molar ratio of aluminium to reagent is 1：1.The linear range of the method is from 0.5 to 120.0μg/L and the detection limit is 0.2μg/L of aluminium.The method has been used to determine trace aluminium in deep-fried dough strip,milk,beer and apricot kernel drink samples with satisfactory results.
关键词
荧光光度法
铝
食品
fluorescence spectrophotometry
aluminium
food
