不孕症患者血清和宫颈粘液AsAb检测及其治疗

Detection of anti-sperm antibodies in serum and cervical mucus of infertile women and its treatment

目的 探讨血清与宫颈粘液抗精子抗体之间的关系及其治疗效果.方法 采用酶联免疫吸附法检测300例不孕症患者血清和宫颈粘液的抗精子抗体,以230例健康妇女作对照组;对抗精子抗体阳性不孕症患者随机分为药物治疗组和安全套法治疗组,药物治疗组给予地塞米松合并维生素治疗,安全套法组仅严格应用安全套.对收集的数据进行统计分析.结果 不孕症组抗精子抗体阳性总计92例,占30.7%,高于对照组的11.3%（χ^2=28.20,P〈0.01）;药物治疗组治愈率为70.2%,安全套法组治愈率为13.3%,两组比较差异有统计学意义（χ^2=30.46,P〈0.01）.结论 测定血清抗精子抗体可间接反映宫颈粘液抗精子抗体的状况;地塞米松合并维生素可有效地消除抗精子抗体.

Objective To study relation between anti-sperm antibodies （AsAb） in serum and those in cervical mucus of infertile women, and to observe therapeutic effect of those infertile women with positive AsAb. Methods Anti-sperm antibodies in serum and cervical mucus of 300 women with infertility were detected with ELISA. 230 healthy women were as controls. Infertile women with positive AsAbs were randomly divided into drug treatment group and condom treatment group. The women in the drug treatment group were treated with dexamethasone （DXM） combined with vitamins C and E, and the women in the condom treatment group only used condoms in their sexul intercourse. The data collected were analyzed statistically. Results 92 patients in infertile group had positive AsAb, and the positive rate was 30.7% , which was higher than in 11.3% in the control group （χ^2 = 28.20, P 〈 0.01 ）. The curative rate was 70.2% in the drug treatment group and 13.3% in the condom treatment group, and there was a significant difference between the two groups （χ^2 = 30.46, P 〈0. 01 ）. Conclusion Anti-sperm antibodies in serum can indirectly reflect the status of anti-sperm antibodies in cervical mucus. DXM combined with vitamins can effectively eliminate AsAbs.