苗药芭蕉体外抗菌活性研究

Antimicrobial Activity of Musa Basjoo In Vitro

目的:研究芭蕉根和芭蕉花不同提取部位对金黄色葡萄球菌(SA)、耐甲氧西林的金黄色葡萄球菌(MRSA)和β-内酰胺酶阳性的金黄色葡萄球菌(ESBLs)的抑菌活性。方法:采用纸片扩散法测定不同提取部位的最低抑菌浓度(MIC)。结果:芭蕉根石油醚部位对SA,MRSA和ESBLs均有抑制作用,MIC全部为31.25μg·disc-1;芭蕉根正丁醇部位对MRSA,ESBLs有抑制作用,MIC都为250μg·disc-1;芭蕉花石油醚部位对SA、MRSA和ESBLs都有抑制作用,MIC均为125μg·disc-1。抑菌活性大小顺序为芭蕉根石油醚部位>芭蕉花石油醚部位>芭蕉根正丁醇部位。结论:芭蕉根和芭蕉花的石油醚部位是芭蕉的抑菌活性部位。

Objective： To study antimicrobial activity against SA,MRSA and ESBLs by Musa basjoo rhizome and flower.Method： Disc diffusion method was applied to determine the MIC value.Result： MIC in the petroleum ether extracts of the rhizome against SA,MRSA and ESBLs was 31.25 μg.disc-1.MIC in n-BuOH extract against MRSA and ESBLs was 250 μg.disc-1.MIC in the petroleum ether extracts of the flower against SA,MRSA and ESBLs was 125 μg.disc-1.The order of antimicrobial activity was petroleum ether extracts of rhizome petroleum ether extracts of flower n-BuOH extract of rhizome.Conclusion： Petroleum ether extracts of rhizome and flower were active fraction.