广西阴香叶挥发油化学成分及其抗氧化性研究

Chemical Constituents and Antioxidant Activity of Essential Oils from Leaves of Cinnamomum burmannii in Guangxi

目的:分析阴香叶Cinnamomum burmannii(Nees)B1.挥发油的化学成分,对挥发油的抗氧化能力进行研究。方法:采用水蒸气蒸馏法提取阴香叶挥发油,用气相色谱-质谱联用技术(GC-MS)分析鉴定其化学成分;采用DPPH.法、ABTS+.法和铁氰化钾还原法对其抗氧化能力进行研究。结果:阴香叶分离出137个色谱峰,共鉴定61个化合物,占挥发油总量的93.58%;DPPH.清除率最高仅为21.19%,清除ABTS+.效率最高达58.89%,还原能力与BHT相比相差较大。结论:阴香叶主要成分为石竹烯(21.71%)、桉油精(18.22%)、愈创醇(7.52%)、(+)-α-萜品醇(7.06%)、(-)-β-蒎烯(3.57%)、γ-桉叶醇(3.33%)、异愈创木醇(3.16%)、(Z)-橙花叔醇(3.16%)、榄香醇(2.67%)、α-石竹烯(2.22%)、(1S)-α-蒎烯(1.90%)、(-)-萜品烯-4-醇(1.80%)、(+)-喇叭烯(1.35%)、石竹烯氧化物(1.29%)、γ-萜品烯(1.05%)等,阴香叶挥发油具有较好ABTS+.清除能力,但清除DPPH.能力及还原能力较弱,其抗氧化能力均与其浓度有关。

Objective：To study the chemical constituents and antioxidant activity of the essential oils from Cinnamomum burmannii in Guangxi.Method： The essential oils were extracted by steam distillation,and then the constituents were separated and identified by GC-MS.The scavenging activities on DPPH radical,ABTS ＋ radical and reducing power were detected by UV-Vis spectrophotometry.Result： 137 compounds were isolated and 61 compounds were identified in the leaves that composed about 93.58% of the total essential oils.The maximal scavenging rate on DPPH and ABTS ＋ radical reached to 21.71% and 58.89%,respectively.And these oils had weaker reductive capability than BHT.Conclusion： The principal chemical constituents of the essential oils are caryophyllene（21.71%）,eucalyptol（18.22%）,guaiol（7.52%）,（＋）-α-terpineol（7.06%）,（-）-β-pinene（3.57%）,γ-eudesmol（3.33%）,bulnesol（3.16%）,（Z）-nerolidol（3.16%）,elemol（2.67%）,α-caryophyllene（2.22%）,（1S）-α-pinene（1.9%）,（-）-terpinen-4-ol（1.8%）,（＋）-ledene（1.35%）,caryophyllene oxide（1.29%）,and γ-terpinen（1.05%）.These oils possessed good scavenging activities on ABTS ＋ radical,but showed low activities on scavenging DPPH radical and reducing power.And the antioxidant activities of these oils were concentration-dependent.