摘要
目的:观察灵芝多糖对试验性衰老大鼠海马内离子型谷氨酸受体-N-甲基-D-天门冬氨酸受体(NMDA受体)2B亚单位(NR2B)表达的影响。方法:健康SD大鼠,雌雄各半,颈背部皮下注射(sc)D-半乳糖(D-gal)制成亚急性衰老模型,用不同浓度的灵芝多糖溶液灌胃(ig)治疗10d,Morris水迷宫试验检测动物学习记忆能力,采用免疫组织化学染色法,WesternBlotting技术检测各组大鼠海马CA1,CA3区NR2B表达的变化。结果:与模型组相比,灵芝多糖125,100 mg.kg-1能显著降低大鼠水迷宫试验的平均潜伏期,升高跨越平台次数及在Ⅳ象限内的游泳时间占整个游泳时间的百分率,亦能显著升高大鼠海马内CA1,CA3区NR2B的表达(P均<0.01)。结论:灵芝多糖能提高试验性衰老大鼠的学习记忆能力;升高此大鼠海马内降低的NR2B表达,可能是其增强大鼠学习记忆的机制之一。
Objective： To explore effects of ganoderma lucidum polysaccharides on expression of hippocampal N-methyl-D-aspartate（NMDA） receptor 2B（NR2B） in aging rats induce by D-gal.Method： The permanent dosage（sc） of D-gal was applied to SD rats to establish the aging model.Different concentrations of Ganoderma lucidum polysaccharides were individually administrated（ig） for 10 days.The Morris water maze was applied to validate the learning in rats.With immunohistochemitry and Western Blotting,the expression of NR2B was detected on tissues of CA1 and CA3 regions in hippocampus in rats.Result： As compared with the model,rats treated with G.lucidum polysaccharides（125,100 mg·kg-1） had longer durations of crossover platform and the persentage of swimming time in Ⅳ quadrant,as well as the increased expression of NR2B in hippocampus（P 0.01,P 0.01）.Conclusion： G.lucidum polysaccharides would play important roles in promoting learning ability for aging rats induced by D-gal.The up-regulation of NR2B expression in hippocampus could be contributed to one of the related mechanisms.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第17期147-150,共4页
Chinese Journal of Experimental Traditional Medical Formulae
