迷走神经切断及烟碱预处理对大鼠全身炎症反应综合征的影响

Preliminary Research on Effect of Pretreatment by Vagotomy and Nicotine on Systemic Inflammatory Reaction Syndrome in Rat

目的:通过复制"两次打击"全身炎症反应综合征(SIRS)大鼠模型,探讨迷走神经切断及烟碱预处理对大鼠SIRS的作用,为临床治疗SIRS提供新思路。方法:选择雄性健康SD大鼠40只,随机分为4组,每组10只。A:对照组未予处理;B:单侧迷走神经切断组;C:行右侧颈部迷走神经切断;烟碱预处理组:每天ip烟碱液5 mg·kg-1;连续7 d;D:联合迷走神经切断+烟碱预处理组。7 d后造模(急性放血+Lps ip);造模后12 h取血检测大鼠外周血白细胞计数与分类、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、乳酸脱氢酶(LDH)、肌酸磷酸激酶(CPK)、尿素氮(BUN)、肌酐(Cr)、谷丙转氨酶(ALT)、谷草转氨酶(AST)以及胆汁酸(TBA)的变化。结果:迷走神经切断后能提高炎症反应;烟碱预处理后能降低炎症反应;联合二者处理后炎症反应介于两者之间。结论:烟碱在次要的胆碱能抗炎通路中的作用不依赖于迷走神经的完整性,可独立发挥作用。

Objective： To investigate the effect of pretreatment by vagotomy and nicotine on systemic inflammatory reaction syndrome（SIRS） in a rat model of ＂two-hit＂,and to provide new ideas for the clinical treatment of SIRS.Method： Forty male SD rats were randomly divided into four groups,i.e.group A（control） without treatment,group B（unilateral vagotomy）,group C（nicotine intervention）,group D（unilateral vagotomy ＋ nicotine intervention）.The above interventions lasted 7 days.Afterwards,group B,group C and group D were treated by hemorrhagic shock and ip LPS for inducing SIPS.Twelve hours later,blood was with drawn to test the blood cell count,TNF-α,IL-6,LDH,CPK BUN,Cr,ALT,AST and TBA.Result： Vagotomy could promote inflammation,and nicotine pretreatment could inhibit inflammation.Joint pretreatment of vagotomy ＋ nicotine could produce and inflammatory state in between.Conclusion：Nicotine in the secondary cholinergic anti-inflammatory pathways appears to be independent to the integrity of vagus nerve.It may function independently.