小儿肺热咳喘口服液防治甲型H1N1流感病毒感染免疫低下小鼠的研究

Preventative and Therapeutical Effect of Xiaoer Feire Kechuan Oral Solution on Immunocompromised Mice Infected by Influenza Virus A (H1N1)

目的:评价小儿肺热咳喘口服液对免疫低下小鼠感染甲型H1N1流感病毒的防治作用。方法:ICR小鼠sc环磷酰胺造成免疫低下,以甲型H1N1流感病毒滴鼻感染造成肺炎模型,造模同时及造模前ig 20,10,5 mL·kg-1小儿肺热咳喘口服液4 d后观察肺部炎症、计算肺指数,死亡率和生命延长率,Real-time RT-PCR法检测肺组织中病毒载量,酶联免疫法检测血清中免疫因子的含量。结果:小儿肺热咳喘口服液治疗性给药或预防性给药后能明显降低流感病毒FM1株和PR8株感染后免疫低下小鼠的肺指数、降低死亡率、延长平均存活天数、显著降低小鼠肺组织中的病毒载量,明显升高小鼠血清中CD4/CD8的比值。结论:小儿肺热咳喘口服液对甲型H1N1流感病毒感染免疫低下小鼠造成的病毒性肺炎具有明显的防治作用。

Objective：To evaluate the preventative and therapeutical effect of Xiaoer Feire Kechuan oral solution on the immunocompromised mice infected by influenza virus A（H1N1）.Method： ICR mice were injected cyclophosphamide to establish immunocompromised mice model,at the same day,the mice were infected by influenza A（H1N1） by nasal drops.The lung index,death rate,death protection rate and life prolongation were observed;The virus load in lungs was detected by real-time RT-PCR and the level of immune factors in blood serum were detected by ELISA.Result： With preventative and therapeutical treatment of Xiaoer Feire Kechuan oral solution,the lung index,death rate and the virus load in lung tissues of immunocompromised mice which were infected by influenza virus FM1 strain and PR8 strain decreased significantly,while the life time of immunocompromised mice was prolonged by the oral solution and CD4 /CD8 ratio in serum increased significantly.Conclusion： Xiaoer Feire Kechuan oral solution could inhibit virus pneumonia in immunocompromised mice caused by influenza virus A（H1N1）significantly.