酒大黄对动脉粥样硬化兔PAI-1及其基因的调节作用

Regulation of Radix et Rhizoma Rhei Prepared with Wine on Expression of PAI-1 mRNA in Aorta in Atherosclerotic Rabbits

目的:研究酒大黄对实验性动脉粥样硬化(AS)兔纤溶酶原激活物抑制物-1(PAI-1)及其基因的调节作用,探讨酒大黄抗AS机制。方法:将健康雄性新西兰兔32只随机分为4组(n=8):空白对照组(N组)、AS模型组(M组)、酒大黄低剂量组(L组)和酒大黄高剂量组(H组),常规高脂饮食构建动脉粥样硬化模型。治疗给药12周,免疫组织化学法检测主动脉组织PAI-1蛋白表达,半定量逆转录聚合酶链反应(RT-PCR)技术检测兔主动脉组织PAI-1 mRNA的表达。结果:与模型组相比,酒大黄低剂量组能显著抑制主动脉组织PAI-1 mRNA以及蛋白的表达(P<0.05或P<0.01);酒大黄高剂量组显著上调主动脉组织PAI-1 mRNA和蛋白的表达(P<0.05或P<0.01)。结论:酒大黄对AS兔PAI-1蛋白及其mRNA表达的调节作用呈现剂量双重性。

Objective：To investigate possible mechanisms of the beneficial effects of Radix et Rhizoma Rhei prepared with wine on the contents and expressions of plasminogen activator inhibitor-1（PAI-1） in atherosclerotic（AS） rabbits induced by high lipid diet.Method： Thirty-two healthy male New-Zealand rabbits were randomly divided into four groups（n = 8 each）： Normal control group（N）,AS model group（M）,low dose（L）and high dose（H）Radix et Rhizoma Rhei prepared with wine groups.After 12-weeks treatment,the protein of PAI-1 was observed by immunohistochemistry method,and PAI-1 mRNA in aortic tissue were detected by RT-PCR method.Result： In low dose group,the expression of PAI-1 mRNA and protein in aortic tissue decreased significantly compared with AS model group（P 0.05 or P 0.01）.In high dose group,the expression of PAI-1 mRNA and protein in aortic tissue were significantly increased compared with the other three groups.（P 0.05 or P 0.01）.Conclusion： Radix et Rhizoma Rhei prepared with wine may play a dual role in controlling of PAI-1 gene expression.