丙肝病毒F基因对c-myc转录调控作用

Effect of HCV-F gene on transcription activation of c-myc promoter

目的观察丙型肝炎病毒（HCV）F基因对c-myc启动子转录活性的影响,探讨HCV F基因表达产物参与肝细胞癌变的机制。方法构建c-myc启动子的萤光素酶报告质粒,与HCV-F和HCV-C的表达质粒共转染人肝癌细胞系HepG 2细胞,测定萤光素酶的活性,分析HCV-F、HCV-C对c-myc启动子转录活性的影响。结果在HepG 2细胞中,HCV-C对c-myc启动子的转录具有明显的促进作用,启动子的转录活性比对照组提高约7倍,且在HCV-C的剂量为2~10μg时,其作用具有剂量依赖性;HCV-F对c-myc启动子转录活性也具有激活作用,在剂量为2μg时,启动子的转录活性约为对照组的3倍,c-myc启动子对HCV-F的剂量依赖激活作用不明显。结论 HCV-F相比HCV-C不能有效的调节c-myc启动子转录活性,HCV-C起主要的调节功能。

Objective To observe the effect of hepatitis C virus-F（HCV-F） gene on transcription activition of c-myc promoter and to explore the role of HCV-F on pathogenesis of hepatocarcinogenesis.Methods The luciferase reporter plasmids of c-myc promoter were constructed and cotransfected into HepG 2 cells with HCV-F and HCV-C expression plasmids.The transactivition of c-myc promoter was assayed by luminometer.Results HCV-C exhibited a distinct promotion activity to c-myc promoter,with a seven-folds increase of c-myc promoter transactivity compared to that of the control group.HCV-F showed less promotion activity and enhanced luciferase activity about three-folds increse of c-myc promoter.At a range of 2 to 10 μg,the enhancement of c-myc promoter was observed to be dose-dependent only with HCV-C not HCV-F.ConclusionHCV-F exhibits less promotion activity for c-myc promoter than HCV-C,which does not posses all functions of HCV-C protein.