期刊文献+

半胱氨酸蛋白酶-3在染砷大鼠肾近端小管表达 被引量:2

Expression of caspase-3 in renal proximal convoluted tubules in rats with arsenic poisoning
原文传递
导出
摘要 目的探讨慢性染砷对大鼠肾脏近端小管上皮细胞半胱氨酸蛋白酶3(caspase-3)表达的影响。方法将30只健康成年清洁级SD大鼠随机分为高、低剂量(分别为10.0,0.4 mg/kg)染砷组和对照(蒸馏水)组,采用自由饮水方式(As2O3溶于饮水中)进行染毒,染毒容量为200 mL/kg,连续染毒6个月,测血砷、尿砷含量,取大鼠肾脏进行HE染色,免疫组化法检测染砷大鼠近端小管上皮细胞caspase-3的表达,图像分析计数。结果与对照组比较,高、低剂量染砷组大鼠血砷、尿砷明显增高(P<0.01);染砷大鼠近端小管上皮细胞空泡样变性,细胞腔面刷状缘脱落并减少,可见核固缩;与对照组(1.30±1.02)个/视野比较,高、低剂量染砷组大鼠肾近端小管上皮细胞caspase-3阳性细胞数分别为(63.94±10.83)、(16.00±8.23)个/视野,明显升高(P<0.05);高、低剂量染砷组大鼠肾近端小管上皮细胞caspase-3表达灰度值分别为(90.43±11.43)、(107.94±10.72),与对照组比较,差异均有统计学意义(P<0.05)。结论慢性砷中毒可致大鼠近端小管上皮细胞caspase-3表达。 Objective To explore the effect of chronic arsenic poisoning on the caspase-3 expression in epithelium cells of proximal convoluted tubules in rats.Methods Thirty healthy adult SD rats in cleanliness grade were divided randomly into three groups:high and low dose arsenism group with arsenism of 10.0 and 0.4 mg/kg·d,respectively,and control group with distilled water.Drinking water was contaminated with As2O3.The rats were exposed to the contaiminated drinking water freely for six months.The content of arsenic in the blood and urine of the rats was determined.The change of configuration and expression of caspase-3 in epithelium cells of proximal convoluted tubules of the kidneys of arsenism rats were observed with HE,strept vidin-biotin complex(SABC) immunohistochemistry and image pattern analysis methods.Results Compared with the control group,a higher arsenic content in the blood and urine was found in both high and low dose arsenism group(P0.01).Vacuole degeneration was found in epithelium cells and brush border of lumen surface fell off and reduced with pyknotic nucleus in proximal convoluted tubules of arsenism rats.The positive cells of caspase-3 was increased in control group(1.30±1.02),low dose(16.00±8.23/area) and high dose(63.94±10.83/area)(P0.05).While the grey scale was decreased significantly in high dose(107.94±10.72/area) and low dose(148.23±11.74/area) compared to that of the control group(P0.05).Conclusion Chronic arsenic poisoning may induce the expression of caspase-3 in epithelium cells in proximal convoluted tubules in rats.
出处 《中国公共卫生》 CAS CSCD 北大核心 2010年第12期1550-1551,共2页 Chinese Journal of Public Health
基金 贵州省科技计划项目(黔科合S字【2007】1059号)
关键词 砷中毒 半胱氨酸蛋白酶3(caspase-3) 近端小管 凋亡 大鼠 arsenic poisoning caspase-3 proximal tubule apoptosis rat
  • 相关文献

参考文献8

二级参考文献35

共引文献53

同被引文献12

  • 1孙晓玲.原发性肝癌患者手术前后血清NO/NOS、TNF检测的临床意义[J].放射免疫学杂志,2005,18(4):271-272. 被引量:7
  • 2车德才,李水仙,武延隽,赵中夫,张芸.急性肝衰竭大鼠血清TNF-α和IFN-γ/IL-4表达[J].中国公共卫生,2007,23(9):1088-1089. 被引量:7
  • 3Romero R, Espinoza J, Chaiworapongsa T, et al. Infection and pre-maturity and the role of preventive strategies[ J]. Semin Neonatol, 2002,7(4) :259 -274.
  • 4Forge A, Schacht J. Aminoglycoside antibiotics [ J ]. Audiol Neuro- otol,2000,5 ( 1 ) :3 -22.
  • 5Willott JF, Hnath CT, Lister JJ. Modulation of presbycusis : current status and future directions [ J ]. Audiol Neurootol,2001,6 ( 5 ) : 231 - 249.
  • 6Watanabe K, Inai S, Jinnouchi K, et al. Expression of caspase- acti- vated deoxyribonuclease (CAD) and caspase-3 ( CPP32 ) in the cochlea of cisplatin (CDDP) -treated guinea pigs [ J ]. Auris Nasus Larynx,2003,30(3) :219 -225.
  • 7Endo Y,Shibazaki M, Yamaguchi K, et al. Enhancement by galac- tosamine of lipopolysaccharide (LPS)-induced tumour necrosis fac-tor production and lethality: its suppression by LPS pretreatment [ J ]. British Journal of Pharmacology, 1999,128 ( 1 ) :5 - 12.
  • 8Diaz-Guerra M,Velasco M,Martin-Sanz P,et al. Nuclear factor κB is required for the transcriptional control of type Ⅱ NO synthase in regenerating liver [ J ]. Biochem J, 1997,326 : 791 - 797.
  • 9Shrivastava A, Tiwari M, Sinha RA, et al. Molecular iodine in- duces caspase-independent apoptosis in human breast carcinoma cells involving the mitochondria-mediated pathway [ J ]. Journal of Biological Chemistry,2006,281 (28) : 19762 - 19771.
  • 10Acehan D, Jiang X, Morgan DG, et al. Three-dimensional structure of the apoptosome: implications for assembly, procaspase-9 binding, and activation[J]. Molecular Ce11,2002,9 (2) :423 -432.

引证文献2

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部