开颖、闭颖小麦浆片的显微观察

Lodicules Microscopic Observation of Noncleistogamy and Cleistogamy Wheat

为了研究小麦开颖、闭颖授粉的原因,为探索小麦开颖、闭颖授粉机理奠定基础。此研究以开颖授粉小麦漯麦4号和闭颖授粉小麦花培3号为材料,在小麦抽穗期取浆片,用冷冻切片法观察两个品种浆片的厚度,并测量浆片的厚度,发现漯麦4号浆片的厚度是花培3号的1.67倍。对浆片进行整体透明处理,用微分干涉差显微镜观察浆片整体外形和浆片的中部细胞,对比高度和宽度,发现两者浆片在高度和宽度上无明显差异;同时对比浆片表面中部细胞的大小,发现两者浆片的中部细胞大小也无明显差异。

Wheat （Triticum aestivum L.） is a self-pollinating crop.But most of wheat is noncleistogamy.Contrasting with noncleistogamy,cleistogamy can keep the purity of pure line varieties,reduce the risk of spike disease infection and insure the seed set under stress environment.In order to find the reason which lead to the noncleistogamy and cleistogamy in wheat （Triticum aestivum L.） and lay the foundation for the exploration of the mechanism in noncleistogamy and cleistogamy.In this study,the noncleistogamy variety ＇ Luomai 4 ＇ and cleistogamy variety ＇ Huapei 3 ＇ were used as materials.The lodicules were taken while the materials were heading.The depths of lodicules were compared through frozen section,its depths were measured as well.The lodicules ＇ depths in ＇ Luomai 4 ＇ were 1.67 times the lodicules ＇ depths in ＇ Huapei 3 ＇.The whole clearing method was also taken to discover highs,widths and cell sizes difference between two varieties.The observation was carried out by the differential interference microscope.It was found that the lodicules ＇ highs and widths between two types （noncleistogamy and cleistogamy） were not difference.The lodicules ＇ cell sizes of two types were also no significantly difference.