秋水仙素诱导巨桉无性系Eg5多倍体的研究

Polyploidy Induction of Clone Eg5 of Eucalyptus grandis with the Use of Colchicine

以巨桉无性系Eg5组培苗的腋芽为处理材料,使用Plackkett-Burman设计和均匀设计安排试验,对秋水仙素诱导巨桉无性系Eg5多倍体的影响因素和诱导率间的关系进行研究。经过回归分析找到了多倍体诱导率和其自变量秋水仙素浓度及浸泡时间的关系式,其AdjR-sq=0.9366,回归效果理想。当秋水仙素浸泡浓度为0.21%、浸泡时间为26.2h时,诱导率可达其最大值51%。在秋水仙素浓度和浸泡时间增加过程中诱导率呈现先增加,后减小的变化趋势。此时,导致诱导率不断降低的一个重要原因是处理芽的大量死亡。

The study was conducted to find the dependency of polyploid inductivity rate of Eucalyptus grandis clones ＇ axillary buds with colchicine on treatment conditions by applying Plackkett-Burman Design and Uniform Design. According to regression analysis, the functional relation equation between polyploid inductivity rate and independent variables including colchicines concentration and soaking time was found whose value of Adj R-sq was 0.9366 and ideal. The maximum of inductivity rate can reach 51% when the soaking time was 26.2 h and the colchicines concentration is 0.21% . The study result showed that the inductivity rises at fist and drops later with the continuous increase of colchicine concentration and soaking time. The inductivity dropped because of the mass mortality of awaiting axillary buds.