抑癌基因PLAGL1在人肝细胞癌中的表达及其意义

Significance and expression of tumor suppressor gene PLAGL1 in human hepatcellular carcinoma

目的 探讨抑癌基因PLAGL1在人肝细胞癌和癌旁组织中的表达及其意义.方法 随机选取肝细胞癌（HCC）30例和远离癌组织的癌旁组织30例,免疫组织化学分析PLAGL1蛋白表达,分析其意义.培养正常肝细胞株L02和肝癌细胞株HepG2细胞,采用逆转录-聚合酶链反应（RT-PCR）方法检测其PLAGL1 mRNA表达水平.探讨与肝癌细胞组织中PLAGL1蛋白表达相关性.结果 PLAGL1蛋白在癌旁组织中表达明显强于肝细胞癌组织,2例（7%）肝细胞癌组织中PLAGL1表达成阳性,27例（90%）癌旁组织中表达成阳性,两组之间PLAGL1的表达水平差异有统计学意义（χ2=38.44,P〈0.05）.RT-PCR结果显示PLAGL1 mRNA在L02细胞中明显高于HepG2细胞.结论 PLAGL1蛋白的表达下调可能与肝细胞癌的发生和发展相关,PLAGL1蛋白表达下降与PLAGL1 mRNA下调相平行.

Objective To study the significance and protein expression of tumor suppressor gene PLAGL1 in humor hepatocellular carcinoma （HCC） and mRNA expression in L02 and HepG2 cells.Methods Thirty cases of HCC tissues and 30 cases of tumor-adjacent tissues were collected from patients surgically treated in our hospital.The protein expression of PLAGL1 was detected by immunohistochemistry.The normal liver cell line L02 and hepatoma cell line HepG2 cells were cultured,and the expression of PLAGL1 mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR）.Results PLAGL1 protein expression in tumor-adjacent tissues （ n = 27,90% ） was significantly stronger than in HCC tissues （n = 2,7% ） （χ2 = 38.44,P 〈 0.05 ）.RT-PCR results showed that PLAGL1 mRNA in the L02 cells was significantly higher than in HepG2 cells.Conclusion Down-regulation of PLAGL1 protein may be correlated to the occurrence and development of HCC.PLAGL1 protein expression was paralleled with the PLAGL1 mRNA down-regulation.