ω-3不饱和脂肪酸对大鼠肝切除术后肠屏障的影响

Effects of ω-3 polyunsaturated fatty acid on gut barrier in rat partial hepatectomy models

目的 观察ω-3不饱和脂肪酸（ω-3PUFA）对大鼠肝切除术后肠屏障的影响.方法 雄性SD大鼠96只随机分为假手术组（S）、生理盐水组（NS＋PH）和大（H＋PH）、小剂量（L＋PH）ω-3PUFA治疗组（2ml/kg、1 ml/kgω-3PUFA静脉注射＋大鼠70%肝切除组）.检测肝切除术后1、2、3、5 d谷丙转氨酶（ALT）、谷草转氨酶（AST）、门静脉血中自细胞介素（IL）-6、肿瘤坏死因子（TNF）-α、内毒素（ET）水平的变化以及小肠黏膜的病理形态学改变.结果ω-3PUFA治疗组术后1、2、3 d ALT、AST较生理盐水组有显著下降（P〈0.05）.术后1、2、3、5 d,ω-3PUFA治疗组门静脉血中TNF-α、IL-6有显著降低,内毒素水平也有显著下降[H＋PH组分别为：（69.61±15.65）、（53.20±6.26）、（35.90±9.76）、（31.81±8.96）ng/L,L＋PH组分别为：（79.99±8.73）、（56.11±5.69）、（43.46±14.39）、（34.40±4.88）ng/L,P〈0.05].病理学检查可见生理盐水组肠上皮黏膜组织形态受损明显,ω-3PUFA治疗组肠上皮黏膜受损较小.结论 围手术期应用ω-3PUFA可能通过减少炎症因子的释放达到保护肠屏障的效果.

Objective To investigate the effects of ω-3 polyunsaturated fatty acid （ω-3PUFA） on gut barrier in rat partial hepatectomy models and explore the possible mechanism.Methods Male Sprague-Dawley rats were randomly divided into sham operation group,partial hepatectomy （NS ＋ PH） group,PH ＋ low-dose PUFA （ L ＋ PH,1 mL/kg） group and PH ＋ high-dose PUFA （ H ＋ PH,2 mL/kg） group.Serum samples of portal vein were collected to measure the levels of interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） at the 1st,2nd,3rd and 5th day after hepatectomy.The plasma levels of endotoxin （ET） and the intestinal mucosa pathological changes were also examined.Results Compared with the PH group,the levels of ET [H ＋ PH： （ 69.61 ± 15.65 ）,（ 53.20 ± 6.26 ）,（ 35.90 ± 9.76 ）,（ 31.81 ±8.96） ng/L,L＋PH： （79.99 ±8.73）,（56.11 ±5.69）,（43.46 ± 14.39）,（34.40 ±4.88） ng/L]and the expression of IL-6 and TNF-α were decreased significantly in the low-and high-dose ω-3PUFA treatment groups after partial hepatectomy （P 〈 0.05 ）.Histological examination revealed the injury of intestinal mucosa in PH group and the rats in ω-3PUFA treated groups showed alleviated severity of ultrastructural injury.Conclusion The supplemenation of ω-3PUFA repaired the injury of gut barrier after partial hepatctomy,which may contribute to the inhibition of inflammatory cytokines expression such as IL-6 and TNF-α.