中华鳖胰岛细胞分离及生物活性检测

Experimental study of separation and bioactivity of Trionyx sinensis islet cells

目的 探讨中华鳖胰岛细胞分离、提取方法及对其功能评价,为临床胰岛移植探索一种新的供体来源.方法 采用胶原酶消化分离中华鳖胰岛细胞,在体外用不同浓度的葡萄糖刺激胰岛细胞,测定其生物学活性.结果中华鳖胰岛细胞在体外对不同浓度的葡萄糖刺激具有明确的生物学反应,并随着葡萄糖浓度的增加胰岛素的产生也相应增加.结论 采用胶原酶消化分离中华鳖胰岛细胞的方法可行,胰岛细胞在体外对不同浓度的葡萄糖刺激产生胰岛素并具有正相关,其为爬行纲与哺乳纲动物之间的胰岛细胞移植提供实验基础.

Objective To explore Trionyx sinensis islet cells separation,extraction method and function evaluation,provide a new donor source for clinical islet transplantation research.Methods The separation of Trionyx sinensis islet cells by collagenase,stimulating islet cells using different concentration glucose in vitro,and determining its bioactivity.Results Trionyx sinensis islet cells show definite biological response to different concentration glucose in vitro,then increase of insulin yield according to increase of glucose concentration.Conclusion The method of separation of Trionyx sinensis islet cells is feasible,the relation between insulin yield from Trionyx sinensis islet cells and different concentration glucose is positive correlation,which establish experimental basis for islet cell transplantation between reptilia and mammalia.