摘要
Drought stress is one of the most important factors limiting maize production. Rab17 is an ABA-responsive gene and associated with drought tolerance. In order to identify haplotypic structure and mine allelic variants at tab17 locus, nucleotide diversity and linkage disequilibrium （LD） structure of rab17 were evaluated among a mini core set of Chinese diversified maize inbred lines. Totally, 19 SNP and 18 insertion/deletions （InDels） were identified, among which 81% were in non-coding regions and 19% in coding regions. The results showed that a high level of diversity appeared within 1 kb upstream of the rabl 7 locus, and declined quickly downstream of the gene region. Rapid decay of linkage disequilibrium of rabl 7 region with distance within 1 kb was detected. Functional markers which can be developed based on haplotype 14 are expected to have contribution to molecular breeding for drought tolerance.
Drought stress is one of the most important factors limiting maize production. Rab17 is an ABA-responsive gene and associated with drought tolerance. In order to identify haplotypic structure and mine allelic variants at tab17 locus, nucleotide diversity and linkage disequilibrium （LD） structure of rab17 were evaluated among a mini core set of Chinese diversified maize inbred lines. Totally, 19 SNP and 18 insertion/deletions （InDels） were identified, among which 81% were in non-coding regions and 19% in coding regions. The results showed that a high level of diversity appeared within 1 kb upstream of the rabl 7 locus, and declined quickly downstream of the gene region. Rapid decay of linkage disequilibrium of rabl 7 region with distance within 1 kb was detected. Functional markers which can be developed based on haplotype 14 are expected to have contribution to molecular breeding for drought tolerance.
基金
supported by the National Basic Research Program of China (973 Program, 2011CB100105)
the National High-Tech R&D Program (863 Program,2006AA10Z188)
the National Natural Science Foundation of China (30730063)
