摘要
建立了鲍鱼的高效液相(HPLC)指纹图谱.研究了不同的预处理方法、流动相组成、最佳检测波长、色谱柱温度等操作因素对鲍鱼指纹谱图的影响,并对不同产地的鲍鱼指纹图谱进行了比较.色谱条件为不锈钢填充色谱柱(Venusil XBP C18,4.6 mm×250 mm,5μm);检测波长为210 nm;流动相为乙腈-体积分数0.1%的磷酸水溶液梯度洗脱;流速0.5 mL/min;分析时间70.00 min;柱温30℃.
To develop a method of discriminating the fingerprint of abalone by HPLC. The different sample pretreatment mobile phase detection wavelength temperature and compared the fingerprint chromatograms of abalone from three different areas were studied. Chromatographic conditions： Column Venusil XBP C18 （4.6 mm× 250 mm, 5 μm） was used with the mixture of acetonitrile-0.1% phosphoric acid; the detection wavelength was 210 nm ;the flow rate was 0.5 mL/min; the time was 70 min and the column temperature was 30℃
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2010年第6期676-681,共6页
Journal of Hebei University(Natural Science Edition)
基金
国家科技支持计划项目(2006BAK02A17-6)
