HPLC法测定孕妇金花片中小檗碱的含量

Quantitative Determination of Paeoniflorin of Yunfujinhua Tablet by HPLC Method

目的：建立孕妇金花片中小檗碱含量测定方法。方法：采用高效液相色谱法,色谱柱为中科院大连化学物理研究所KF色谱柱,流动相为甲醇-乙腈-磷酸（0.1%）溶液（10∶30∶60）,检测波长为346nm。结果：小檗碱在0.192~0.96g之间线性关系良好（r=0.99937,n=5）,回收率为105.37%,RSD为1.06%。结论：该方法操作简单,分离效果好,灵敏度高,可作为孕妇金花片的质量控制标准。

Objective：A HPLC method for the determination of berberine of Yunfujinhua tablet was established.Methods：A KF chromatographic column was used with solution of methyl alcohol-acetonitrile-phosphoric acid（10∶30∶60） as the mobile phase,The detection wavelength was 346nm.Results：The linear range was0.192～0.96g,（r=0.99937）and average rate of recovery was 105.37%（RSD =1.06%）.Conclusion：The method is simple and sensitive with good separation efficiency,it can be used in the standard of quantitative determination of Yunfujinhua tablet.