低能N^+注入选育黑曲霉β-葡萄糖苷酶高产菌株及其发酵优化研究

Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N^+ implantation

为了获得β-葡萄糖苷酶高产菌株,应用低能N+注入技术对黑曲霉菌株Au进行了诱变选育,同时研究了N+注入对菌株存活率和突变率的影响。通过几轮注入之后,最终筛选得到的高产菌株Au 0847其β-葡萄糖苷酶活力达到13.75 U/mL,比原始菌株Au提高了106.8%,且遗传性能稳定。经过进一步的发酵优化,该菌株最终β-葡萄糖苷酶活力达到30.53 U/mL。

Low-energy N＋ implantation was employed to mutate Aspergillus niger Au to enhance productivity of β-glucosidase.Effects of N＋ on strains,survival and mutation rate were studied.After several rounds of implantation,activity of β-glucosidase of the final mutant Au 0847 reached 13.75 U/mL,which is higher by 106.8% than that of original strain Au,and its heritability was stabilized.Activity of β-glucosidase of Au 0847 reached 30.53 U/mL after further fermentation condition optimization.