慢性鼻-鼻窦炎患者细菌生物膜表达与细菌培养的相关性研究

Relationship between bacterial biofilm and bacterial culture in patients with chronic rhinosinusitis

目的 探讨细菌生物膜在慢性鼻-鼻窦炎（chronic rhinosinusitis,CRS）患者鼻黏膜中的表达与细菌培养的相关性.方法 获取90例CRS患者的手术黏膜样本,并以5例鼻中隔偏曲患者及10名健康志愿者的钩突黏膜样本作为对照.所有样本均行细菌培养及扫描电子显微镜检查,细菌生物膜表达与细菌培养的相关性分析采用Pearson检验.结果 90例实验组患者中,64例观察到细菌生物膜,阳性率为71.1%,其中60例（66.7%）患者细菌培养阳性.实验共检出61株细菌,其中检出率最高的为表皮葡萄球菌,占41.0%（25/61）.对照组及其余26例实验组患者均未观察到细菌生物膜,也未培养出需氧或厌氧菌.细菌培养与细菌生物膜间的Pearson相关系数为0.901（P=0.000）.结论 CRS患者鼻黏膜中细菌生物膜表达与细菌培养阳性结果高度相关.

Objective To investigate the relationship between bacteria biofilm and bacterial culture in patients with chronic rhinosinusitis （CRS）. Methods Ninety patients with CRS were enrolled in the study. Five patients with deviation of nasal septum and 10 healthy subjects served as controls. Mucosa of uncinate process or near the ostium of the maxillary sinus was obtained during endoscopic sinus surgery. All specimens were processed for bacterial culture and scanned by electron microscopy. Pearson test was performed to analyze the relationship between the presence of bacterial biofilm and the results of bacteria culture. Results The scanning electron microscopy showed bacterial biofilms in 64 （71.1%） out of 90patients with CRS, while the positive rate of bacteria culture in the study group was 66.7% （60/90）. No bacterial biofilm and bacterium was detected in the control group and 26 culture-negative individuals in study group. Pearson correlation analysis showed a statistically association between bacterial biofilm and bacterial culture in CRS （ r = 0. 901, P = 0. 000）. Conclusion Positive results of bacteria culture are highly correlated with the presence of bacterial biofilm in CRS patients.