多药耐药大肠埃希菌抗菌制剂外排泵基因研究

Efflux pumps in multidrug resistant Escherichia coli

目的 探讨多药耐药大肠埃希菌（multidrug-resistant Escherichia coli,MDR-ECO）的抗菌制剂外排泵基因流行状况.方法 收集临床样本中分离的20株MDR-ECO,采用聚合酶链反应（PCR）法检测11种抗菌制剂外排泵基因：emrB、emrD、emrE、mdfA、sugE、mdtI、tehA、oqxA、qacE△1、qacE和smr-2.结果 本组MDR-ECO检出emrB、emrD、emrE、mdfA、sugE、mdtI、qacE△1、tehA和oqxA 9种质子泵,多数菌株能同时检出多种外排泵基因,且发现同时携带8种外排泵基因的大肠埃希菌.结论 临床分离MDR-ECO可能与细菌携带多种抗菌制剂外排泵基因相关.

Objective To explore the prevalence of 11 efflux pumps in isolates of multidrugresistant Escherichia coli（MDR-ECO）. Methods Efflux pumps emrB, emrD , emrE, mdfA, sugE, mdtl,tehA, oqxA, qacE△1, qacE and smr-2 were detected by polymerase chain reaction（PCR）in 20 MDR-ECOs isolated from clinical samples. Results Efflux pumps emrB, emrD, emrE, mdfA, sugE, mdtI, qacE △1, tehA and oqxA were detected, and 8 efflux pumps were found in the same strain. Conclusion Multidrug- resistance in Escherichia coli may be related with efflux pumps.