重楼、水蛭药对改善血黏度及对肺癌转移的影响

Effects of Compatibility of Rhizoma Paridis and Hirudo on Metastasis of Lung Cancer Through Improving Blood Viscosity

目的:观察解毒中药重楼配伍活血中药水蛭对肺癌小鼠肺转移灶和血黏度的影响。方法:采用C57BL/6小鼠Lew is肺癌模型,分为正常组、模型组、化疗组、重楼+水蛭组、重楼组、水蛭组,每组8只;各组药物治疗后检测肿瘤肺转移灶个数和全血低切黏度、全血高切黏度、红细胞聚集指数。结果:重楼+水蛭组与模型组相比,肺转移率显著降低(P<0.01),较重楼组和水蛭组差异也有统计学意义(P<0.05,P<0.01)。与模型组相比,重楼+水蛭组和水蛭组的全血低切黏度、高切黏度显著降低(P<0.05),重楼+水蛭组的红细胞聚集指数明显下降(P<0.05)。结论:重楼配伍水蛭能显著抑制小鼠肺癌模型的肺转移发生率,其机制跟改善血黏度有关;合理运用活血中药能有助于肺癌的治疗。

Objective： To observe the effects of compatibility of Rhizoma Paridis and Hirudo on metastasis of lung cancer and blood viscosity in mice with lung cancer.Methods： Lewis lung caner models of C57BL/6 mice were randomly divided into 6 groups： the normal group（A）,the model group（B）,the chemotherapy group（C）,Rhizoma Paridis ＋ Hirudo group（D）,Rhizoma Paridis group（E）,and Hirudo group（F）,with 8 mice in each group.After treatment,the number of lung cancer metastasis,whole blood low shear viscosity,whole blood high shear viscosity and aggregation index of red blood cells（RBC） were observed.Results： The number of lung cancer metastasis in group D was significantly lower than that in group B,group E and group F（P0.05 or P0.01）.The whole blood low shear viscosity and whole blood high shear viscosity in group D and group F and the aggregation index of RBC in group D were obviously decreased compared with group B（P0.05）.Conclusion： The compatibility of Rhizoma Paridis and Hirudo can inhibit the metastasis of lung cancer in Lewis lung caner models of C57BL/6 mice and its mechanism may have connection with blood viscosity,so the application of blood-activating herb can help treat lung cancer.