五鹤续断对辐射损伤小鼠保护作用的研究

Study on radioprotective effect of Wu-He Dipsacus asper in mice

目的 研究五鹤续断（WHDA）注射液对辐射损伤小鼠的保护作用.方法 将160只昆明种雌性小鼠分为正常对照组、照射组和低剂量WHDA＋照射组、高剂量WHDA＋照射组,观察其30 d存活率;测定辐射后6 h、3 d、6 d、9 d小鼠的外周血象;测定辐射后小鼠的胸腺、脾脏和肝脏质量;测定辐射后小鼠外周血和肝脏的超氧化物歧化酶（SOD）、谷胱甘肽（GSH）及丙二酰二醛（MDA）水平.结果 WHDA可提高辐射后小鼠的存活率,对其白细胞及血小板的数量变化有显著影响,但对其胸腺、脾脏、肝脏质量均无明显影响.WHDA还能显著提高辐射后小鼠的SOD活性,增加GSH水平,降低MDA水平.结论 WHDA对辐射损伤小鼠有明显的保护作用.

Objective To study protective effect of Wu-He Dipsacus asper（WHDA） injection on the radiation damaged mice. Methods One hundred and twenty Kunming female mice were divided into normal control group, model control group, Wu-He Dipsacus asper treatment group to observe the 30-day survival rate;measured peripheral blood 6 hours, 3 days, 6 days and 9 days after irradiation, respectively; thymus, spleen and liver weight were detected; superoxide dismutase （SOD）, glutathione （GSH）, malonyldialdehyde （MDA） in liver and peripheral blood were also detected. Results WHDA can improve the survival rate of mice after radiation, and take a significant influence on white blood cells and platelets, but no obvious effect on the weight of thymus, spleen and liver. In addition, WHDA can improve SOD activity, increased GSH content and decrease MDA content significantly in this study. Conclusion WHDA has obvious protective effect in mice with radiation injury.