摘要
目的:采用复合磷脂脂质体技术制备莪术油脂质体并研究其最佳制备工艺及处方。方法:采用高效液相色谱法和分光光度法分别测定复合磷脂脂质体中莪术醇和莪术油的含量。采用乙醇注入法制备莪术油复合磷脂脂质体,并以脂质体中药物含量为考察指标,采用正交试验优选了莪术油复合磷脂脂质体的最优处方与工艺。结果:以优化的工艺和处方制备的莪术油复合磷脂脂质体,包封率稳定,药物含量高。结论:该脂质体制备方法准确可靠,工艺可行,质量稳定可控。
Objective:To investigate the preparation of the novel liposome composed of hydrogenated soybean phosphatidylcholine ( HSPC ) and soybean phosphatidylcholine ( SPC ) containing zedoary turmeric oil ( ZTO). Method : HPLC analysis and spectrophotometry analysis were used to determine the contents of curcumol and ZTO in ZTO liposomes both composed of HSPC and SPC respectively. The ZTO novel liposomes were prepared by ethanol injection method. The best prescription of ZTO liposome both composed of HSPC and SPC was determined through orthogonal design, using the drug content of liposomes as the evaluation index. Result: When ZTO novel liposome was prepare with the best prescription, both the entrapment efficiency stability and the drug content were high. Conclusion: The method was accurate and applicable. The preparation was feasible and the quality of the novel liposome was stable and controllable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期14-17,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
南京中医药大学引进人才启动项目(200802)