HPLC测定猴耳环不同部位中没食子酸和槲皮素的含量

HPLC Determination of Gallic Acid and Quercetin in Different Parts of Archidendron clypearia

目的:测定猴耳环药材不同部位没食子酸和槲皮素的含量,为合理开发利用猴耳环资源提供理论依据。方法:采用高效液相法,色谱条件:Kromosil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸溶液梯度洗脱,流速1 mL.min-1,检测波长254 nm。结果:没食子酸和槲皮素的含量均是在叶中最高,幼枝中次之,茎干中含量最低,茎干中没有检测出槲皮素。结论:HPLC简便可行、重复性好,适用于猴耳环2种成分的同时测定,可用于猴耳环的质量控制。

Objective：To determine gallic acid and quercetin from different parts of Archidendron clypearia, and to provide evidence for the rational development and utilization of A. clypearia. Method： HPLC method was used with Kromosil ClsCOlumn （4.6 mm × 250 mm, 5 μm） , and the mobile phases were acetonitrile and 0.2% phosphoric acid solution with the gradient elution. The flow rate was 1 mL. min^-1 and the detection wavelength was 254 nm. Result： Both of the content of gallic acid and quercetin are highest in the leaves,lower in the new shoot and lowest in the shoot, even quercetin cannot be tested in the shoot. Conclusion： The method can be used to control the quality of Archidendron clypearia. It is simple, accurate, reproducible and suitable for simultaneous determination of two active constituents in Archidendron clypearia.