摘要
目的:利用代谢组学的方法研究不同剂量CCl4诱导的小鼠肝损伤模型,确定能够表征CCl4肝损伤模型的血浆代谢物中的生物标记物。方法:采用基于超高效液相色谱-飞行时间质谱(UPLC-TOF-MS)的代谢组学技术,并结合主成分分析的方法,研究正常小鼠和不同剂量CCl4诱导的肝损伤小鼠血浆代谢物组的变化。结果:初步确定建立小鼠肝损伤血浆代谢物研究模型的CCl4用量,并寻找到10个具有特征性的内源性生物标记物。结论:代谢组学方法成功用于CCl4诱导的小鼠血浆肝损伤模型研究,并为进一步研究中药的保护肝脏作用奠定了基础。
Objective：Use the metabonomics method to study the CCl4-induced liver injury model,and discriminate the potential biomarkers contributed to liver injury in plasma metabolites.Method：Adopt the metabolic skills based on UPLC-TOF-MS and PCA,to research the change of plasma metabolite in normal rat or CCl4-induced liver injury rat with two different doses.Result： Ascertain the CCl4 dosage in establish rat liver injury model of plasma metabolites and find ten characteristic biomakers in plasma.Conclusion： Metabonomics method was successfully used to study the CC14-induced liver injury model by the rat plasma, and laid a foundation for the further study of the liver protection of Traditional Chinese Medicine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期98-101,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金资助项目(81001599)
科技部重大新药创制(2009ZX09103-339)
江苏省中医药管理局研究项目(LB09031)
南京中医药大学青年自然科学基金项目(09XZR14)
