四物汤及其组方药对与药味对小鼠原发性痛经模型的影响

Studys on Influence of Siwu Decoction and its Composite Drug-pairs or Drugs on Primary Dysmenorrheal Model of Mouse

目的:研究四物汤及其组方药对、药味对原发性痛经模型小鼠的影响,探讨其组方特点及可能作用机制。方法:采用小鼠原发性痛经模型,观察各给药组对原发性痛经模型小鼠的扭体反应以及子宫组织匀浆中钙离子(Ca2+)、一氧化氮(NO)水平的影响。结果:四物汤组方药对、药味能不同程度地减少痛经模型小鼠的扭体反应次数,降低子宫组织中Ca2+含量,提高NO水平。结论:四物汤及其拆方对小鼠原发性痛经模型的干预作用各有特点,其中熟地对其他3味药在抑制整体动物模型的扭体反应上可能具有拮抗作用,当归-白芍药对可能是抑制小鼠原发性痛经模型的主要贡献药对,为进一步深入研究提供了参考。

Objective：To investigate the influence of Siwu decoction and its composite drug-pairs or drugs on the primary dysmenorrheal model of mouse so as to clarify the potential mechanism and compatible regularity of Siwu decoction.Method： The model of primary dysmenorrheal mice were used to investigate the effect of Siwu decoction on the writhing response and the levels of nitric oxide and calcium ion in mice uterine tissue.Result：Siwu decoction and its composite drug-pairs and drugs had certain pharmacological effect on primary dysmenorrheal by reducing uterine tissue writhing times, increasing nitric oxide （NO） levels and reducing calcium ion （ Ca^2 ＋ ） concentration in of mice. Conelusion：Siwu decoction and its composite drug-pairs and drugs exhibited characteristic influence on the primary dysmenorrheal model of mouse. The drug of Rehmanniae Radix Praeparata might have the antagonistic effect to Chuanxiong Rhizoma, Angelicar Sinensis Radix and Paeoniae Radix Alba on the activity of reducing the model mice＇ s writhing times. The drug-pair of Angelicar Sinensis Radix and Paeoniae Radix Alba may be the most significant drug-pair for the treatment effect on the model of primary dysmenorrheal mice. This study provided a reliable reference for the follow-up study.