正交优选苗药验方隔梨胃炎消提取工艺

Optimization of Extraction Techniques for Geli Weiyanxiao by Orthogonal Experiment

目的:优选苗药验方治疗慢性胃炎的提取工艺。方法:选取水和乙醇作为提取溶媒,以溶剂浓度、溶剂用量、提取时间、提取次数及药材粉碎粒度作为考察因素,每个因素设立3个水平;并以提取物的镇痛、抗炎作用,浸出物为指标,利用L9(34)正交设计优选提取工艺。结果:水提最佳提取工艺为粉碎粒度为粗颗粒,加10倍量水,提取1 h,提取3次;醇提最佳提取工艺为粉碎粒度为最粗粉,加10倍量乙醇,提取2 h,乙醇浓度为70%,提取2次。考虑到节约成本以及考察指标浸出物、镇痛率、抗炎率的结果显示水提组均优于醇提组;最终确定提取工艺为水提,药材粉碎粒度为粗颗粒,加10倍量水,提取1 h,提取3次。结论:优选的提取工艺稳定,提取物药效显著。

Objective：To optimize the extraction techniques of Geli Weiyanxiao. Method：The selection of optimum proess was investigated by L9 （3^4） orthogonal design with the Geli Weiyanxiao as the observation index. Result：Water extraction process： to smash the best size for coarse particles, plus 10 times the volume of water, from 1 hour, 3 times. Ethanol extraction process： to smash the best size for 10 mesh number, addingl0-fold 70% ethanol, eatracting for 2 times, 2 hours for each extraction ; In consideration of cost-saving and the results of various indexes, the optimal process condition was selected as follows： the best size for coarse particles, plus 10 times the volume of water, from 1 hour, 3 times. Conclusion：The optimal extraction technique for Geli Weiyanxiao is stable and reliable.