扶正固本颗粒联合化疗放疗对于荷瘤小鼠的免疫增强作用

Immue-improving Effect of Fuzheng Guben Granule Combined with Radiotherapy and Chemotherapy on Tumor-bearing Mice

目的:研究扶正固本颗粒联合化疗、放疗的体内抗肿瘤作用及对荷瘤小鼠免疫功能的影响,并与单独放疗、化疗作比较。方法:建立小鼠肉瘤S180移植性肿瘤模型,次日随机分为10组,放、化疗,并ig给药每日1次,10 d后,计算抑瘤率、胸腺指数和脾指数,测定细胞因子白介素-2(IL-2),白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平和外周血细胞数量,检测其抑瘤作用及对荷瘤小鼠免疫功能的影响。结果:联合治疗较单独的放化疗能增强荷瘤小鼠腹腔巨噬细胞吞噬功能,提高荷瘤小鼠的胸腺指数和脾脏指数;拮抗化疗所致荷瘤小鼠血清IL-2,IL-6和TNF-α的减少;拮抗放疗所致荷瘤小鼠外周血细胞的减少。结论:联合治疗可增强荷瘤小鼠免疫功能。

Objective ： To investigate the anti-tumor and immune effect of Fuzheng Guben Granule combined with chemotherapy and radiotherapy in tumor-bearing mice. Method ：Tumor-bearing model of S180 sarcoma in mice was established by subcutaneous injection of S-180 ascites at armpit. On the next day, the mice were randomly divided into 10 groups, Fuzheng Guben Granule with radio- and chemotherapy was carried out once a day for ten days. Tumor inhibitory percentage, thymus gland, spleen indexes were then measured. The determination of eytokine, such as IL-2,IL-6,TNF-α and peripheral blood cell counts was made. The effect of treatment on tumor inhibition and immunological function was studied. Result：The combined therapy could significantly increase thymus gland,spleen indexes and improved the phagocytic function; antagonize serum IL-2, IL-6 and TNF-α reduction induced by chemotherapy; suppress the decrease in peripheral blood cells count induced by radiotherapy. Conclusion：Combinative therapy can enhance immune functions for S180-bearing mice.