汉黄芩素对人肺腺癌SPC-A-1细胞增殖和周期的影响

Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells

目的:研究汉黄芩素对人肺腺癌SPC-A-1细胞增殖和周期的影响。方法:以不同浓度的汉黄芩素作用于SPC-A-1细胞,检测细胞抑制率、凋亡及细胞周期。结果:MTT法测得汉黄芩素的IC50为24.7 mg.L-1,能够明显抑制SPC-A-1细胞的集落形成能力。汉黄芩素作用于SPC-A-1细胞后,荧光倒置显微镜下观察到细胞凋亡的形态学改变。Annexin V-FITC/PI双标法证实汉黄芩素可以诱导SPC-A-1细胞凋亡,并具有剂量依赖性。流式细胞仪结果显示,不同浓度汉黄芩素使SPC-A-1细胞周期出现显著的变化,10,20 mg.L-1汉黄芩素可将周期阻滞在G0/G1期,30,40 mg.L-1汉黄芩素则使细胞周期堆积在S期,并且随着给药剂量的增加,凋亡峰显著升高。结论:汉黄芩素能明显抑制SPC-A-1细胞增殖,诱导细胞凋亡,并对细胞G0/G1和S期具有阻滞效应,具有抗肿瘤作用。

Objective：To investigate the effect of wogonin on the proliferation and cell cycle of the SPC-A-1 cell line.Method： The cultured SPC-A-1 cells were treated with different concentrations of wogonin.The proliferation of SPC-A-1 cells was detected by MTT method.Cell apoptosis and cell cycle were detected by AO ＋ EB straining and flow cytometry.Result：After wogonin administration,SPC-A-1 cells proliferation inhibition ratio and apoptosis ratio increased significantly in a dose-dependent manner.AO ＋ EB straining showed partial cells presented characteristic morphological changes of apoptosis. SPC-A-1 cells showed G0/G1 phase blockage when the concentration of wogonin was 10,20 mg· L^-1, and S phase blockage appeared when the concentration of wogonin was 30, 40 mg·L^-1. Conclusion ： Wogonin can inhibit the proliferation of SPC-A-1 cells and induce apoptosis, also possess the effect of G0/G1 and S phase blockage and potential antitumor effect.